Xue Dongsheng, Yao Donghai, You Xihuo, Gong Chunjie
1Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, 430068 People's Republic of China.
China Agricultural Huawei Biopharmaceuticals Ltd Company, Wuxue, 435300 People's Republic of China.
Indian J Microbiol. 2020 Jun;60(2):175-181. doi: 10.1007/s12088-020-00856-9. Epub 2020 Jan 9.
The green synthesis of the flavor esters, -propyl acetate, isobutyl acetate and isoamyl acetate had the advantages over the chemical synthesis. The esterase from could transform -propanol, isobutanol and isoamyl alcohol into -propyl acetate, isobutyl acetate and isoamyl acetate, respectively. The esterase was expressed in . At 30 °C for 1 d, the concentration of -propyl acetate, isobutyl acetate and isoamyl acetate synthesized by the esterase expressed in was 24.6 mg/100 mL, 8.3 mg/100 mL, 5.6 mg/100 mL, respectively. Expression of the esterase has a practical significance for flavor ester synthesis by green biochemical process.
风味酯类物质乙酸丙酯、乙酸异丁酯和乙酸异戊酯的绿色合成比化学合成具有优势。来自[具体来源未明确]的酯酶可分别将丙醇、异丁醇和异戊醇转化为乙酸丙酯、乙酸异丁酯和乙酸异戊酯。该酯酶在[具体表达宿主未明确]中表达。在30℃下作用1天,由在[具体表达宿主未明确]中表达的酯酶合成的乙酸丙酯、乙酸异丁酯和乙酸异戊酯的浓度分别为24.6mg/100mL、8.3mg/100mL、5.6mg/100mL。酯酶的表达对于通过绿色生化过程合成风味酯具有实际意义。