Bahmani Kiumars, Shahbazi Yasser, Nikousefat Zahra
1Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.
Food Sci Biotechnol. 2019 Aug 26;29(3):441-448. doi: 10.1007/s10068-019-00665-x. eCollection 2020 Mar.
A total of 450 samples consisting of red meat, poultry meat, aquatic product and raw milk were collected during winter 2016 and summer 2017. 22.2% (100/450) of collected meat and raw milk samples were found to be contaminated with antibiotic residues in the initial screening using Premitest. According to the enzyme linked immunosorbent assay (ELISA) results, the mean tetracyclines (TCs) concentration of meat samples determined as follows: chicken (155.41 µg/kg) > turkey (138.68 µg/kg) > quail (130.7 µg/kg) > cow (108.92 µg/kg) > calf (105.18 µg/kg) > goat (99.4 µg/kg) > sheep (95.22 µg/kg) > rainbow trout (35.62 µg/kg) > shrimp (31.80 µg/kg). The content of TCs in cow, goat and sheep milk samples were found to be ranged 45.6-163.5 µg/L, 72.4-101.1 µg/L and 65.5-98.9 µg/L, respectively. 5.7% (26/450) of samples had TCs confirmed the ELISA results using high performance liquid chromatography coupled with ultra-violet detection, although the concentration of TCs residues in samples was higher than that of ELISA.
2016年冬季和2017年夏季共采集了450份包括红肉、禽肉、水产品和生乳的样本。在使用普瑞来检测进行的初步筛查中，发现22.2%（100/450）的采集肉和生乳样本被抗生素残留污染。根据酶联免疫吸附测定（ELISA）结果，肉类样本中四环素（TCs）的平均浓度如下：鸡肉（155.41μg/kg）＞火鸡（138.68μg/kg）＞鹌鹑（130.7μg/kg）＞奶牛（108.92μg/kg）＞小牛（105.18μg/kg）＞山羊（99.4μg/kg）＞绵羊（95.22μg/kg）＞虹鳟鱼（35.62μg/kg）＞虾（31.80μg/kg）。奶牛、山羊和绵羊奶样本中TCs的含量分别为45.6 - 163.5μg/L、72.4 - 101.1μg/L和65.5 - 98.9μg/L。尽管样本中TCs残留浓度高于ELISA检测结果，但仍有5.7%（26/450）的样本通过高效液相色谱-紫外检测法确认了ELISA结果。
