Haque Shaila, Jamil Md Yusuf, Khan Md Shahinul Haque, Reza Md Sajib Al, Esrafil Md, Abedin Md Zainul, Zubair Md Abu, Sikder Md Asaduzzaman, Bari Luthfunnesa
Department of Food Technology and Nutritional Science, Mawlana Bhashani Science and Technology University, Tangail, Bangladesh.
Department of Chemistry, Bangladesh University of Health Science, Dhaka, Bangladesh.
Front Antibiot. 2024 Apr 3;3:1364946. doi: 10.3389/frabi.2024.1364946. eCollection 2024.
The misuse of antibiotics in poultry farming is a global issue.
The focus of this study was the health risk assessment of consumers from the determination of ciprofloxacin (CIP), tetracycline (TC), and oxytetracycline (OTC) in broiler chicken in the raw, frozen, and boiled stages using solid-phase extraction, high-performance liquid chromatography, and ultraviolet detection (SPE-HPLC-UV).
Chromatographic separation was achieved using 0.3% metaphosphoric acid and acetonitrile (1:10, /) for CIP at 280 nm and oxalic acid (0.01 M) and acetonitrile (1:1, /) for TC and OTC at 355 nm with different retention times. The method had an acceptable precision with good linearity, specificity, limit of detection, limit of quantification, accuracy, and stability.
Among a total of 252 raw samples, approximately 68.25%, 25.4%, and 7.54% contained CIP, TC, and OTC, respectively. Out of the positive raw samples, CIP exceeded the maximum residual limit (MRL) in 3.6% muscle, 14.3% liver and 17.9% skin samples, whereas TC and OTC were below the MRLs. The residual concentrations of these antibiotics were almost unchanged in frozen samples. After boiling the chicken samples, the TC and OTC residues were reduced significantly compared to CIP. Although the concentrations of CIP in boiled samples were above the MRL set by the European Union, these did not exceed the hazard index 1.
Based on these results, the exposure levels to antibiotics in broiler chicken meats may be considered to have a low risk for human health.
家禽养殖中抗生素的滥用是一个全球性问题。
本研究的重点是通过固相萃取、高效液相色谱和紫外检测（SPE-HPLC-UV）测定生、冻、煮阶段肉鸡中环丙沙星（CIP）、四环素（TC）和土霉素（OTC），对消费者的健康风险进行评估。
采用0.3%偏磷酸和乙腈（1:10，v/v）在280 nm波长下对CIP进行色谱分离，采用0.01 M草酸和乙腈（1:1，v/v）在355 nm波长下对TC和OTC进行色谱分离，保留时间不同。该方法具有可接受的精密度，线性、特异性、检测限、定量限、准确度和稳定性良好。
在总共252份生样品中，分别约有68.25%、25.4%和7.54%含有CIP、TC和OTC。在阳性生样品中，CIP在3.6%的肌肉、14.3%的肝脏和17.9%的皮肤样品中超过最大残留限量（MRL），而TC和OTC低于MRL。这些抗生素在冷冻样品中的残留浓度几乎没有变化。将鸡肉样品煮沸后，与CIP相比，TC和OTC残留量显著降低。虽然煮沸样品中CIP的浓度高于欧盟设定的MRL，但这些浓度未超过危害指数1。
基于这些结果，可认为食用肉鸡中抗生素的暴露水平对人类健康风险较低。
