Kang Ju-Bin, Park Dong-Ju, Koh Phil-Ok
Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828 South Korea.
Lab Anim Res. 2019 Nov 26;35:24. doi: 10.1186/s42826-019-0026-9. eCollection 2019.
Glutamate leads to neuronal cell damage by generating neurotoxicity during brain development. The objective of this study is to identify proteins that differently expressed by glutamate treatment in neonatal cerebral cortex. Sprague-Dawley rat pups (post-natal day 7) were intraperitoneally injected with vehicle or glutamate (10 mg/kg). Brain tissues were isolated 4 h after drug treatment and fixed for morphological study. Moreover, cerebral cortices were collected for protein study. Two-dimensional gel electrophoresis and mass spectrometry were carried out to identify specific proteins. We observed severe histopathological changes in glutamate-exposed cerebral cortex. We identified various proteins that differentially expressed by glutamate exposure. Identified proteins were thioredoxin, peroxiredoxin 5, ubiquitin carboxy-terminal hydrolase L1, proteasome subunit alpha proteins, isocitrate dehydrogenase, and heat shock protein 60. Heat shock protein 60 was increased in glutamate exposed condition. However, other proteins were decreased in glutamate-treated animals. These proteins are related to anti-oxidant, protein degradation, metabolism, signal transduction, and anti-apoptotic function. Thus, our findings can suggest that glutamate leads to neonatal cerebral cortex damage by regulation of specific proteins that mediated with various functions.
谷氨酸在脑发育过程中通过产生神经毒性导致神经元细胞损伤。本研究的目的是鉴定新生大鼠大脑皮层中经谷氨酸处理后差异表达的蛋白质。将出生7天的Sprague-Dawley大鼠幼崽腹腔注射溶剂或谷氨酸（10mg/kg）。药物处理4小时后分离脑组织并固定用于形态学研究。此外，收集大脑皮层用于蛋白质研究。采用二维凝胶电泳和质谱法鉴定特定蛋白质。我们观察到谷氨酸处理的大脑皮层出现严重的组织病理学变化。我们鉴定出多种经谷氨酸处理后差异表达的蛋白质。鉴定出的蛋白质有硫氧还蛋白、过氧化物酶体增殖物激活受体5、泛素羧基末端水解酶L1、蛋白酶体亚基α蛋白、异柠檬酸脱氢酶和热休克蛋白60。热休克蛋白60在谷氨酸处理条件下增加。然而，其他蛋白质在谷氨酸处理的动物中减少。这些蛋白质与抗氧化、蛋白质降解、代谢、信号转导和抗凋亡功能有关。因此，我们的研究结果表明，谷氨酸通过调节具有多种功能的特定蛋白质导致新生大鼠大脑皮层损伤。
