Trauma Center/Department of Emergency and Trauma Surgery, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
Plastic and Aesthetic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Biomed Res Int. 2020 Feb 28;2020:9152140. doi: 10.1155/2020/9152140. eCollection 2020.
Pyroptosis has been known to play a vital role in the inflammation process which was induced by infection, injury, or inflammatory disease. The present study was aimed at evaluating the percentage of peripheral blood mononuclear cell (PBMC) pyroptosis in septic patients and assessing the correlation of PBMC pyroptosis with the severity and the mortality of septic patients.
128 trauma-induced patients with sepsis were enrolled in this prospective cohort study. Blood samples were collected, and PBMC pyroptosis was measured by flow cytometry within 24 hours after sepsis was diagnosed.
Percentage of PBMC pyroptosis was positively correlated with the acute physiology and chronic health evaluation (APACHE) II score and sequential organ failure assessment (SOFA) score (all < 0.01). The area under the curve (AUC) for the percentage of PBMC pyroptosis on a receiver operating characteristic curve was 0.79 (95% confidence interval (CI), 0.68-0.90). A Cox proportional hazard model identified an association between an increased percentage of PBMC pyroptosis (>14.17%) and increased risk of the 28-day mortality (hazard ratio = 1.234, 95% CI, 1.014-1.502).
The percentage of PBMC pyroptosis increases in septic patients, and the increased percentage of PBMC pyroptosis is associated with the severity of sepsis and the 28-day mortality of patients with sepsis.
细胞焦亡已被证实在感染、损伤或炎症性疾病引起的炎症过程中发挥重要作用。本研究旨在评估脓毒症患者外周血单个核细胞（PBMC）焦亡的百分比，并评估 PBMC 焦亡与脓毒症患者严重程度和死亡率的相关性。
本前瞻性队列研究纳入了 128 例创伤后发生脓毒症的患者。在诊断脓毒症后 24 小时内采集血样，并通过流式细胞术测量 PBMC 焦亡。
PBMC 焦亡的百分比与急性生理学和慢性健康评估（APACHE）Ⅱ评分和序贯器官衰竭评估（SOFA）评分呈正相关（均<0.01）。PBMC 焦亡百分比的受试者工作特征曲线下面积（AUC）为 0.79（95%置信区间（CI），0.68-0.90）。Cox 比例风险模型确定 PBMC 焦亡百分比增加（>14.17%）与 28 天死亡率增加之间存在关联（风险比=1.234，95%CI，1.014-1.502）。
脓毒症患者的 PBMC 焦亡百分比增加，且 PBMC 焦亡百分比增加与脓毒症的严重程度和脓毒症患者的 28 天死亡率相关。
