Department of Plastic Surgery, Tongji Hospital, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Trauma Center/Department of Emergency and Traumatic Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Dis Markers. 2022 Feb 8;2022:5176915. doi: 10.1155/2022/5176915. eCollection 2022.
DDX3X acts as the critical checkpoint of death in stressed cells. The purpose of this study was to evaluate the mRNA expression level of DDX3X in T cells in peripheral blood of patients with sepsis and to explore its correlation with the prognosis of sepsis.
Seventy-nine patients with traumatic sepsis were enrolled in this prospective cohort study. Blood samples were collected within 24 hours after the diagnosis of sepsis or septic shock, and the mRNA expression level of DDX3X in T cells was detected by PCR.
The level of DDX3X mRNA in T cells was significantly increased in septic patients as well as in septic shock patients. The level of DDX3X mRNA was negatively correlated with T cell count and positively correlated with acute physiological and chronic health assessment (APACHE) score and sequential organ failure assessment (SOFA) score ( < 0.01). The area under the curve (AUC) of the receiver operating characteristic (ROC) curve was 0.79 (95% confidence interval (CI), 0.68-0.90). A Cox proportional hazard model identified an association between an increased DDX3X mRNA level (≥1.575) and the risk of 28-day mortality (hazard ratio = 9.540, 95% CI, 2.452-37.108).
High level of DDX3X mRNA in T cells in sepsis is associated with the severity of sepsis and the mortality of patients with sepsis.
DDX3X 作为应激细胞死亡的关键检查点。本研究旨在评估脓毒症患者外周血 T 细胞中 DDX3X 的 mRNA 表达水平，并探讨其与脓毒症预后的相关性。
本前瞻性队列研究纳入了 79 例创伤性脓毒症患者。在诊断为脓毒症或脓毒性休克后 24 小时内采集血样，并通过 PCR 检测 T 细胞中 DDX3X 的 mRNA 表达水平。
脓毒症患者和脓毒性休克患者 T 细胞中 DDX3X mRNA 的水平显著升高。DDX3X mRNA 水平与 T 细胞计数呈负相关，与急性生理和慢性健康评估（APACHE）评分和序贯器官衰竭评估（SOFA）评分呈正相关（<0.01）。受试者工作特征（ROC）曲线下面积（AUC）为 0.79（95%置信区间，0.68-0.90）。Cox 比例风险模型确定 DDX3X mRNA 水平升高（≥1.575）与 28 天死亡率的风险相关（危险比=9.540，95%置信区间，2.452-37.108）。
脓毒症患者 T 细胞中高水平的 DDX3X mRNA 与脓毒症的严重程度和患者的死亡率相关。
