Pistoresi-Palencia M C, Galmarini M, Vottero-Cima E
Cátedra de Inmunología y Serología, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
J Reprod Immunol. 1988 Dec;14(3):257-65. doi: 10.1016/0165-0378(88)90025-3.
In a previous work, we showed that the immunization of male rats, 3 and 12 months old, with saline extract of rat male accessory glands chemically modified (MRAG) and human serum albumin (HSA) induced a higher humoral and cellular autoimmune response in old animals than in young ones. We have also demonstrated that the facilitation of the autoimmune response is transferred by spleen total cells of 12-month-old animals. The immune response to HSA was not modified. In this work, the cellular type involved in such facilitation was analyzed. For this transference experiment, cells enriched in T and B lymphocytes and macrophages were used. The results showed that the macrophage is the main cellular type involved. However, the transference was only total with the three cellular types together. The study, performed with macrophages pulsed in vivo with MRAG-HSA and then transferred to normal recipients, indicated that although the macrophages from young and old animals were capable of presenting the antigens, the latter did this with significantly greater efficiency for the autoantigen.
在之前的一项研究中，我们发现，用化学修饰的大鼠雄性附属性腺盐水提取物（MRAG）和人血清白蛋白（HSA）对3月龄和12月龄的雄性大鼠进行免疫接种后，老年动物比幼年动物产生了更高的体液和细胞自身免疫反应。我们还证明，12月龄动物的脾全细胞可传递自身免疫反应的促进作用。对HSA的免疫反应未发生改变。在这项研究中，分析了参与这种促进作用的细胞类型。对于此转移实验，使用了富含T淋巴细胞、B淋巴细胞和巨噬细胞的细胞。结果表明，巨噬细胞是主要参与的细胞类型。然而，只有三种细胞类型一起转移时才会完全实现转移。对用MRAG-HSA在体内进行脉冲处理然后转移至正常受体的巨噬细胞进行的研究表明，尽管来自幼年和老年动物的巨噬细胞都能够呈递抗原，但老年动物的巨噬细胞呈递自身抗原的效率明显更高。
