Esther Lydia D, Khusro Ameer, Immanuel P, Esmail Galal Ali, Al-Dhabi Naif Abdullah, Arasu Mariadhas Valan
PG Food Chemistry and Food Processing, Loyola College, Nungambakkam, Chennai 600034, India.
Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai 600034, India.
J Photochem Photobiol B. 2020 Apr 1;206:111868. doi: 10.1016/j.jphotobiol.2020.111868.
Yoghurt is a fermenting milk-based dairy product that has high nutritional benefits. It exhibits not only protection against osteoporosis but also enhances gut microbiota and aids digestion. In order to improve health beneficial aspects of yoghurt, this study was aimed to synthesize gold nanoparticles (AuNPs) using seeds oil of pomegranate (Punica granatum L.) and to formulate functional yoghurt for its antioxidant and anticancer properties. The synthesized AuNPs were characterized using UV-Vis spectrophotometer, FT-IR, XRD, EDX, SEM, DLS, and Zeta potential analyzer. The photo-induced synthesis of AuNPs showed particle size and zeta potential of 70 nm and +34 mV, respectively, with unique peak at 525 nm as observed using UV-Vis spectrophotometer. The FT-IR spectrum of AuNPs showed shifts in the functional groups from 3632.27 to 541.899 cm, thereby indicating the presence of various functional groups in pomegranate seed oil (PSO) and PSO-capped AuNPs. The AuNPs were observed to be smooth, elongated, and rectangular in shape. The PSO-capped AuNPs based formulation of functional yoghurt revealed DPPH degradation (23.6 ± 1.5 to 62.5 ± 1.8%) and HO scavenging traits (21.6 ± 1.3 to 62.8 ± 1.8%) at varied concentrations. In addition, the PSO-capped AuNPs depicted strong anticancer attributes against lung and colon cancer with the cell viability ranging from 80.3 to 25% and 83.3 to 28.4.2%, respectively. Results concluded that the antioxidative components of PSO might have reduced and formulated AuNPs-based functional yoghurt. This functional yoghurt may reveal pivotal applications in food, nutraceuticals, and pharmaceuticals, especially as antioxidant and anticancer agents.
酸奶是一种发酵的奶制品，具有很高的营养价值。它不仅能预防骨质疏松，还能改善肠道微生物群并有助于消化。为了提升酸奶对健康有益的方面，本研究旨在利用石榴（Punica granatum L.）籽油合成金纳米颗粒（AuNPs），并制备具有抗氧化和抗癌特性的功能性酸奶。使用紫外可见分光光度计、傅里叶变换红外光谱仪、X射线衍射仪、能量散射X射线光谱仪、扫描电子显微镜、动态光散射仪和zeta电位分析仪对合成的AuNPs进行了表征。通过光诱导合成的AuNPs，其粒径和zeta电位分别为70nm和+34mV，用紫外可见分光光度计观察到在525nm处有独特的峰值。AuNPs的傅里叶变换红外光谱显示官能团从3632.27cm移动到541.899cm，从而表明石榴籽油（PSO）和PSO包覆的AuNPs中存在各种官能团。观察到AuNPs形状光滑、细长且呈矩形。基于PSO包覆的AuNPs制备的功能性酸奶在不同浓度下显示出二苯基苦味酰基自由基（DPPH）降解（从23.6±1.5%降至62.5±1.8%）和羟基自由基（HO）清除特性（从21.6±1.3%降至62.8±1.8%）。此外，PSO包覆的AuNPs对肺癌和结肠癌表现出很强的抗癌属性，细胞活力分别为80.3%至25%和83.3%至28.4.2%。结果表明，PSO的抗氧化成分可能还原并制备了基于AuNPs的功能性酸奶。这种功能性酸奶可能在食品、营养保健品和制药领域有重要应用，特别是作为抗氧化剂和抗癌剂。
