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利用动态微流控拉曼技术检测前列腺癌细胞中的脂肪酸摄取。

Fatty-Acid Uptake in Prostate Cancer Cells Using Dynamic Microfluidic Raman Technology.

机构信息

Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.

School of Chemical Engineering and Analytical Science, University of Manchester, Manchester M13 9PL, UK.

出版信息

Molecules. 2020 Apr 3;25(7):1652. doi: 10.3390/molecules25071652.

DOI:10.3390/molecules25071652
PMID:32260207
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7180971/
Abstract

It is known that intake of dietary fatty acid (FA) is strongly correlated with prostate cancer progression but is highly dependent on the type of FAs. High levels of palmitic acid (PA) or arachidonic acid (AA) can stimulate the progression of cancer. In this study, a unique experimental set-up consisting of a Raman microscope, coupled with a commercial shear-flow microfluidic system is used to monitor fatty acid uptake by prostate cancer (PC-3) cells in real-time at the single cell level. Uptake of deuterated PA, deuterated AA, and the omega-3 fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were monitored using this new system, while complementary flow cytometry experiments using Nile red staining, were also conducted for the validation of the cellular lipid uptake. Using this novel experimental system, we show that DHA and EPA have inhibitory effects on the uptake of PA and AA by PC-3 cells.

摘要

已知膳食脂肪酸（FA）的摄入与前列腺癌的进展密切相关，但高度依赖于 FA 的类型。高水平的棕榈酸（PA）或花生四烯酸（AA）可刺激癌症的进展。在这项研究中，采用一种独特的实验装置，包括拉曼显微镜和商用剪切流微流控系统，在单细胞水平上实时监测前列腺癌细胞（PC-3）对脂肪酸的摄取。使用该新系统监测氘代 PA、氘代 AA 以及 ω-3 脂肪酸二十二碳六烯酸（DHA）和二十碳五烯酸（EPA）的摄取，同时还进行了使用尼罗红染色的互补流式细胞术实验，以验证细胞脂质摄取。使用这种新型实验系统，我们表明 DHA 和 EPA 对 PC-3 细胞对 PA 和 AA 的摄取具有抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5002/7180971/9536dd3ca616/molecules-25-01652-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5002/7180971/3342a1db1ff4/molecules-25-01652-g0A5a.jpg
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