Bonke Astrid, Sieuwerts Sander, Petersen Iben Lykke
Department of Food Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark.
Brannatura ApS, Nygaardsvej 100, 8660 Skanderborg, Denmark.
Foods. 2020 Apr 3;9(4):429. doi: 10.3390/foods9040429.
Plant-based drinks (PBDs) as alternatives to milk is a fast-growing market in much of the western world, with the demand increasing every year. However, most PBDs from a single plant ingredient do not have an amino acid profile that matches human needs. Therefore, this study set out to combine plant ingredients to achieve a more balanced amino acid profile of novel plant drinks, by combining a high content of oat with the pulses pea () and lentil () in a solution. After removal of the sediment, the resulting plant drinks were composed of what could be kept in suspension. The amino acid and protein composition of the plant drinks were investigated with capillary electrophoresis, to identify the amino acids, and SDS-PAGE to assess the proteins present. The amino acid profile was compared against recommended daily intake (RDI). It was determined that the plant drinks with only oat and lentil did not have a strong amino acid profile, likely due to the higher pH of the lentil concentrate affecting which proteins could be kept in solution. Plant drinks with a combination of both lentil and pea, or only pea, added to the oat drink had an improved concentration of the amino acids that were otherwise in the low end compared to RDI. This includes a high content of phenylalanine, leucine and threonine, as well as a moderate amount of isoleucine, valine and methionine, and a contribution of histidine and lysine. An assessment of stability and sensory parameters was also conducted, concluding there was an advantage of combining oat with a legume, especially pea.
植物基饮料(PBDs)作为牛奶的替代品,在西方世界的大部分地区都是一个快速增长的市场,需求逐年增加。然而,大多数单一植物成分的植物基饮料的氨基酸谱并不符合人体需求。因此,本研究旨在通过将高含量的燕麦与豌豆和小扁豆等豆类在溶液中混合,来组合植物成分,以实现新型植物饮料更平衡的氨基酸谱。去除沉淀物后,所得植物饮料由可保持悬浮状态的成分组成。用毛细管电泳研究植物饮料的氨基酸和蛋白质组成,以鉴定氨基酸,并采用SDS-PAGE评估其中存在的蛋白质。将氨基酸谱与推荐每日摄入量(RDI)进行比较。结果发现,仅含燕麦和小扁豆的植物饮料的氨基酸谱不强,可能是由于小扁豆浓缩物的pH值较高,影响了哪些蛋白质能够保持在溶液中。在燕麦饮料中添加小扁豆和豌豆的组合或仅添加豌豆的植物饮料,其氨基酸浓度相对于RDI有了改善,而这些氨基酸在其他情况下处于较低水平。这包括高含量的苯丙氨酸、亮氨酸和苏氨酸,以及适量的异亮氨酸、缬氨酸和蛋氨酸,还有组氨酸和赖氨酸的贡献。还对稳定性和感官参数进行了评估,得出燕麦与豆类(尤其是豌豆)混合有优势的结论。