The Porcine Nasal Microbiota with Particular Attention to Livestock-Associated Methicillin-Resistant in Germany-A Culturomic Approach.

Livestock-associated methicillin-resistant (LA-MRSA) remains a serious public health threat. Porcine nasal cavities are predominant habitats of LA-MRSA. Hence, components of their microbiota might be of interest as putative antagonistically acting competitors. Here, an extensive culturomics approach has been applied including 27 healthy pigs from seven different farms; five were treated with antibiotics prior to sampling. Overall, 314 different species with standing in nomenclature and 51 isolates representing novel bacterial taxa were detected. was isolated from pigs on all seven farms sampled, comprising ten different types with t899 ( = 15, 29.4%) and t337 ( = 10, 19.6%) being most frequently isolated. Twenty-six MRSA (mostly t899) were detected on five out of the seven farms. Positive correlations between MRSA colonization and age and colonization with , and a negative correlation between colonization with MRSA and spp. were found ( < 0.05). Of 209 non- members of the family, 25 isolates (12.0%) from three out of the seven farms exhibited methicillin resistance, including two isolates carrying the gene. Among 125 , none tested positive for extended-spectrum beta-lactamase (ESBL) and carbapenemase production. The high frequency of methicillin-resistant staphylococci supports the need for enhanced efforts within the "One Health" concept to manage the antibiotic resistance crisis in the human and veterinary medicine sector.