Shi Saige, Zhu Xianglong, Zhao Zengxia, Fang Weijun, Chen Mei, Huang Yizhuan, Chen Xiaolan
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
J Mater Chem B. 2013 Feb 28;1(8):1133-1141. doi: 10.1039/c2tb00376g. Epub 2013 Jan 7.
In this work, we have demonstrated that mesoporous silica-coated Pd@Ag nanoparticles (Pd@Ag@mSiO) can be used as an excellent nanoplatform for photodynamic therapy (PDT) drug delivery. Photosensitizer molecules, Chlorin e6 (Ce6), are covalently linked to the mesoporous shell and the prepared Pd@Ag@mSiO-Ce6 nanoparticles exhibit excellent water solubility, good stability against leaching and high efficiency in photo-generating cytotoxic singlet oxygen. More importantly, the photothermal effect of Pd@Ag nanoplates under the irradiation of a NIR laser can enhance the uptake of Pd@Ag@mSiO-Ce6 nanoparticles by cells, further increasing the PDT efficiency toward cancer cells. The photothermally enhanced PDT effects were demonstrated both in vitro and in vivo. When the Pd@Ag@mSiO-Ce6 nanoparticles were injected intratumorally into the S180 tumor-bearing mice, the tumors were completely destroyed without recurrence of tumors upon irradiation with both 808 nm and 660 nm lasers, while the irradiation with 808 nm or 660 nm alone did not. These results indicate that the Pd@Ag@mSiO nanoparticles may be a valuable new tool for application in cancer phototherapy.
在本研究中，我们证明了介孔二氧化硅包覆的Pd@Ag纳米颗粒（Pd@Ag@mSiO）可作为光动力疗法（PDT）药物递送的优良纳米平台。光敏剂分子二氢卟吩e6（Ce6）与介孔壳层共价连接，制备的Pd@Ag@mSiO-Ce6纳米颗粒表现出优异的水溶性、良好的抗浸出稳定性以及高效产生细胞毒性单线态氧的能力。更重要的是，近红外激光照射下Pd@Ag纳米片的光热效应可增强细胞对Pd@Ag@mSiO-Ce6纳米颗粒的摄取，进一步提高对癌细胞的光动力治疗效率。光热增强的光动力治疗效果在体外和体内均得到了证实。当将Pd@Ag@mSiO-Ce6纳米颗粒瘤内注射到荷S180肿瘤小鼠体内时，在用808 nm和660 nm激光照射后肿瘤被完全破坏且无肿瘤复发，而单独用808 nm或660 nm激光照射则不能。这些结果表明，Pd@Ag@mSiO纳米颗粒可能是用于癌症光疗的一种有价值的新工具。
