Suspène Clément, Piro Benoit, Reisberg Steeve, Pham Minh-Chau, Toss Henrik, Berggren Magnus, Yassar Abderrahim, Horowitz Gilles
Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
J Mater Chem B. 2013 Apr 21;1(15):2090-2097. doi: 10.1039/c3tb00525a. Epub 2013 Mar 6.
This paper reports on the sensing of proteins using water-gated organic field-effect transistors. As a proof-of-concept, streptavidin and avidin were used, with a biotinylated polymer as the active sensing material. The latter is a copolythiophene modified to graft biotin by peptidic coupling. After characterization of its structure, it was integrated as the channel material into transistors and its interactions with several proteins were investigated. Non-specific interactions were reduced when the polymer surface was pretreated with 1-octanol. In this case, human serum albumin had no effect on the transistor characteristics whereas avidin and streptavidin led to a decrease of the drain current.
本文报道了使用水门控有机场效应晶体管对蛋白质的传感研究。作为概念验证，使用了链霉亲和素和抗生物素蛋白，以生物素化聚合物作为活性传感材料。后者是一种通过肽偶联修饰以接枝生物素的共聚噻吩。在对其结构进行表征后，将其作为沟道材料集成到晶体管中，并研究了它与几种蛋白质的相互作用。当聚合物表面用1-辛醇预处理时，非特异性相互作用减少。在这种情况下，人血清白蛋白对晶体管特性没有影响，而抗生物素蛋白和链霉亲和素导致漏极电流下降。
