Sauro Salvatore, Osorio Raquel, Fulgêncio Rogerio, Watson Timothy F, Cama Giuseppe, Thompson Ian, Toledano Manuel
Biomaterials, Biomimetics and Biophotonics (B3), King's College London Dental Institute, Guy's Dental Hospital, King's College and St. Thomas' Hospital, London SE1 9RT, UK.
J Mater Chem B. 2013 May 28;1(20):2624-2638. doi: 10.1039/c3tb00205e. Epub 2013 Apr 23.
Innovative dental restorative materials should be highly bioactive to induce therapeutic effects at the bonding interface during intimate contact with biological fluids. This study aimed at evaluating the remineralisation properties of innovative light-curable resin-based dental materials containing bioactive calcium-phosphosilicates micro-fillers. The apatite formation ability was assessed by ATR-FTIR, XRD, calcium-chelation dye-assisted confocal microscopy (CLSM) and SEM analysis after soaking in SBF. Changes in transition glass temperature (T), water sorption/solubility, alkalinising activity (pH) Knoop micro-hardness (KHN), were also evaluated. Four experimental resin-based materials containing various silicate-base micro-fillers and a control filler-free resin blend were formulated. Disc-shaped specimens were polymerised and submitted to pH/alkalinising activity and water sorption/solubility analyses. The bioactivity and the apatite precipitation induced by the tested materials were evaluated through ATR/FTIR vibrational analysis, SEM and XRD analyses subsequent to SBF storage. Knoop micro-hardness (KHN) and differential scanning calorimetry (DSC) were also performed. The experimental resins containing the bioactive micro-fillers were able to induce apatite precipitation subsequent to prolonged SBF storage (30-90 days). However, the resins containing the bioactive ZnO/polycarboxylated micro-fillers (BAG-Zn; βTCS-Zn) showed the lowest water sorption and solubility mass changes (P < 0.05). A significant increase of the KHN and T subsequent prolonged SBF storage was also observed. The use of such light-curable bioactive materials in restorative dentistry might represent a potentially therapeutic approach to increase the longevity of the restorations via apatite deposition the mineral-depleted tissues at the bonding.
创新的牙科修复材料应具有高生物活性，以便在与生物流体密切接触时在粘结界面诱导治疗效果。本研究旨在评估含有生物活性钙磷硅酸盐微填料的创新光固化树脂基牙科材料的再矿化特性。在模拟体液（SBF）中浸泡后，通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X射线衍射（XRD）、钙螯合染料辅助共聚焦显微镜（CLSM）和扫描电子显微镜（SEM）分析评估磷灰石形成能力。还评估了转变玻璃温度（T）、吸水率/溶解度、碱化活性（pH）、努氏显微硬度（KHN）的变化。配制了四种含有不同硅酸盐基微填料的实验性树脂基材料和一种不含填料的对照树脂混合物。将圆盘形试样聚合，并进行pH/碱化活性和吸水率/溶解度分析。通过SBF储存后的ATR/FTIR振动分析、SEM和XRD分析评估测试材料诱导的生物活性和磷灰石沉淀。还进行了努氏显微硬度（KHN）和差示扫描量热法（DSC）分析。含有生物活性微填料的实验性树脂在长时间SBF储存（30-90天）后能够诱导磷灰石沉淀。然而，含有生物活性氧化锌/多羧化微填料（BAG-Zn；βTCS-Zn）的树脂显示出最低的吸水率和溶解度质量变化（P<0.05）。在长时间SBF储存后，还观察到KHN和T显著增加。在修复牙科中使用这种光固化生物活性材料可能代表一种潜在的治疗方法，通过在粘结处的矿质耗尽组织中沉积磷灰石来提高修复体的寿命。
