Bergoglio Matteo, Najmi Ziba, Cochis Andrea, Miola Marta, Vernè Enrica, Sangermano Marco
Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Torino, Italy.
Department of Health Sciences, Center for Translational Research on Autoimmune and Allergic Diseases-CAAD, Università Del Piemonte Orientale (UPO), 28100 Novara, Italy.
Polymers (Basel). 2023 Oct 14;15(20):4089. doi: 10.3390/polym15204089.
In this study, a bio-based acrylate resin derived from soybean oil was used in combination with a reactive diluent, isobornyl acrylate, to synthetize a composite scaffold reinforced with bioactive glass particles. The formulation contained acrylated epoxidized soybean oil (AESO), isobornyl acrylate (IBOA), a photo-initiator (Irgacure 819) and a bioactive glass particle. The resin showed high reactivity towards radical photopolymerisation, and the presence of the bioactive glass did not significantly affect the photocuring process. The 3D-printed samples showed different properties from the mould-polymerised samples. The glass transition temperature T showed an increase of 3D samples with increasing bioactive glass content, attributed to the layer-by-layer curing process that resulted in improved interaction between the bioactive glass and the polymer matrix. Scanning electron microscope analysis revealed an optimal distribution on bioactive glass within the samples. Compression tests indicated that the 3D-printed sample exhibited higher modulus compared to mould-synthetized samples, proving the enhanced mechanical behaviour of 3D-printed scaffolds. The cytocompatibility and biocompatibility of the samples were evaluated using human bone marrow mesenchymal stem cells (bMSCs). The metabolic activity and attachment of cells on the samples' surfaces were analysed, and the results demonstrated higher metabolic activity and increased cell attachment on the surfaces containing higher bioactive glass content. The viability of the cells was further confirmed through live/dead staining and reseeding experiments. Overall, this study presents a novel approach for fabricating bioactive glass reinforced scaffolds using 3D printing technology, offering potential applications in tissue engineering.
在本研究中，一种源自大豆油的生物基丙烯酸酯树脂与反应性稀释剂丙烯酸异冰片酯结合使用，以合成用生物活性玻璃颗粒增强的复合支架。该配方包含丙烯酸化环氧大豆油（AESO）、丙烯酸异冰片酯（IBOA）、光引发剂（Irgacure 819）和生物活性玻璃颗粒。该树脂对自由基光聚合表现出高反应性，并且生物活性玻璃的存在并未显著影响光固化过程。3D打印样品显示出与模塑聚合样品不同的性能。玻璃化转变温度T显示3D样品随着生物活性玻璃含量的增加而升高，这归因于逐层固化过程，该过程导致生物活性玻璃与聚合物基体之间的相互作用得到改善。扫描电子显微镜分析揭示了生物活性玻璃在样品中的最佳分布。压缩测试表明，与模塑合成样品相比，3D打印样品表现出更高的模量，证明了3D打印支架增强的力学性能。使用人骨髓间充质干细胞（bMSCs）评估样品的细胞相容性和生物相容性。分析了细胞在样品表面的代谢活性和附着情况，结果表明在含有较高生物活性玻璃含量的表面上细胞具有更高的代谢活性和增加的细胞附着。通过活/死染色和再接种实验进一步证实了细胞的活力。总体而言，本研究提出了一种使用3D打印技术制造生物活性玻璃增强支架的新方法，在组织工程中具有潜在应用。
