Xiong Wei-Wei, Yang Guo-Hai, Wu Xing-Cai, Zhu Jun-Jie
State Key Lab. of Analytical Chemistry for Life Science, Key Lab. of Mesoscopic Chemistry of MOE, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
J Mater Chem B. 2013 Sep 7;1(33):4160-4165. doi: 10.1039/c3tb20638f. Epub 2013 Jul 9.
Low-toxicity, highly luminescent, and water-soluble AgInS/ZnS nanocrystals (NCs) have been synthesized via a microwave-assisted approach. The structure and optical features of the AgInS/ZnS NCs were characterized by X-ray diffraction, high resolution transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet visible absorption spectroscopy and photoluminescence (PL) spectroscopy. The as-synthesized AgInS/ZnS NCs exhibited high PL quantum yields (40%) and long PL lifetimes (424.5 ns). Furthermore, the dynamic changes of the intracellular copper(ii) levels in HeLa cells were monitored using the AgInS/ZnS NCs as fluorescent probes. The results showed that the AgInS/ZnS NCs as promising fluorescent probes can be used in the detection of intracellular copper ions in living cells.
通过微波辅助方法合成了低毒性、高发光性且水溶性的AgInS/ZnS纳米晶体（NCs）。采用X射线衍射、高分辨率透射电子显微镜、傅里叶变换红外光谱、紫外可见吸收光谱和光致发光（PL）光谱对AgInS/ZnS NCs的结构和光学特性进行了表征。所合成的AgInS/ZnS NCs表现出高的PL量子产率（40%）和长的PL寿命（424.5 ns）。此外，以AgInS/ZnS NCs作为荧光探针监测了HeLa细胞内铜（II）水平的动态变化。结果表明，AgInS/ZnS NCs作为有前景的荧光探针可用于活细胞内铜离子的检测。
