Fu Libing, Zhuang Junyang, Lai Wenqiang, Que Xiaohua, Lu Minghua, Tang Dianping
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Ministry of Education & Fujian Province), Department of Chemistry, Fuzhou University, 350108 Fuzhou, P.R. China.
J Mater Chem B. 2013 Nov 28;1(44):6123-6128. doi: 10.1039/c3tb21155j. Epub 2013 Oct 9.
A portable and quantitative monitoring protocol for sensitive detection of lead ions is designed, based on target-responsive cargo release from Pb-specific DNAzyme-capped mesoporous silica nanoparticles (MSNs), by coupling with a widely accessible personal glucose meter (PGM). Initially, glucose molecules are loaded into the pores of the MSNs, the pores are then capped with Pb-specific DNAzymes. Upon target introduction, the molecular gates open, resulting in release of the cargo from the pores. The released glucose can be quantitatively monitored using a portable PGM. Under optimal conditions, the as-prepared sensing platform presents good analytical properties for the determination of the target Pb ions, and allows detection of Pb at concentrations as low as 1.0 pM. Importantly, the portable sensing platform has the advantages of simple, on-site, user-friendly and low-cost assessment and has tremendous potential for quantitative detection of non-glucose targets by the public.
基于从铅特异性脱氧核酶封端的介孔二氧化硅纳米颗粒（MSN）中通过与广泛使用的个人血糖仪（PGM）耦合实现目标响应性货物释放，设计了一种用于灵敏检测铅离子的便携式定量监测方案。最初，将葡萄糖分子加载到MSN的孔中，然后用铅特异性脱氧核酶封闭这些孔。引入目标后，分子门打开，导致货物从孔中释放。释放的葡萄糖可以使用便携式PGM进行定量监测。在最佳条件下，所制备的传感平台对目标铅离子的测定具有良好的分析性能，能够检测低至1.0 pM浓度的铅。重要的是，该便携式传感平台具有简单、现场、用户友好和低成本评估的优点，并且在公众对非葡萄糖目标进行定量检测方面具有巨大潜力。
