Zhang JingJing, Lan Tian, Lu Yi
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
GlucoSentient, Inc., 2100 S. Oak Street, Suite 101, Champaign, IL 61820, USA.
Trends Analyt Chem. 2020 Mar;124. doi: 10.1016/j.trac.2019.115782. Epub 2019 Dec 23.
There is a growing demand for high-performance point-of-care (POC) diagnostic technologies where in vitro diagnostics (IVD) is fundamental for prevention, identification, and treatment of many diseases. Over the past decade, a shift of IVDs from the centralized laboratories to POC settings is emerging. In this review, we summarize recent progress in translating IVDs from centralized labs to POC settings using commercially available handheld meters. After introducing typical workflows for IVDs and highlight innovative technologies in this area, we discuss advantages of using commercially available handheld meters for translating IVDs from centralized labs to POC settings. We then provide comprehensive coverage of different signal transduction strategies to repurpose the commercially-available handheld meters, including personal glucose meter, pH meter, thermometer and pressure meter, for detecting a wide range of targets by integrating biochemical assays with the meters for POC testing. Finally, we identify remaining challenges and offer future outlook in this area.
对高性能即时检验（POC）诊断技术的需求日益增长，其中体外诊断（IVD）对于许多疾病的预防、识别和治疗至关重要。在过去十年中，体外诊断正从集中式实验室向即时检验环境转移。在本综述中，我们总结了使用商用手持式血糖仪将体外诊断从集中式实验室转化为即时检验环境的最新进展。在介绍了体外诊断的典型工作流程并强调该领域的创新技术之后，我们讨论了使用商用手持式血糖仪将体外诊断从集中式实验室转化为即时检验环境的优势。然后，我们全面介绍了不同的信号转导策略，通过将生化检测与用于即时检验的血糖仪集成，来重新利用商用手持式血糖仪（包括血糖仪、pH计、温度计和压力计）检测广泛的目标。最后，我们确定了该领域仍然存在的挑战并展望了未来前景。
