Mn-doped NaYF:Yb/Er upconversion nanoparticles with amplified electrogenerated chemiluminescence for tumor biomarker detection.

Chemiluminescence was used as an excitation light source to construct a universal photoelectrochemical platform based on Mn-doped NaYF:Yb/Er upconversion nanoparticles, which greatly improves the electrochemiluminescence intensity and offers more stable cathodic signals compared to pure NaYF:Yb/Er NPs. Here, we report for the first time the ECL behaviors of Mn-doped NaYF:Yb/Er NPs, which were synthesized via a facile strategy. Mn doping resulted in a 4-fold ECL intensity enhancement of NaYF:Yb/Er. The characteristics of Mn-doped NaYF:Yb/Er nanocomposites were obtained using transmission electron microscopy (TEM), energy dispersive X-ray spectrometer (EDS), and fluorescence spectra. After all the results had indicated that NaYF:Yb/Er upconversion nanoparticles were successfully doped with Mn, the electrochemiluminescence platform was built. The as-prepared NaYF:Yb/Er NPs were rendered water-soluble and then employed as ECL emitters for carcinoembryonic antigen (CEA) determination. The results indicated that the modified electrode can be used to determine CEA without interference from non-specific proteins, with a low detection limit of 5.2 pg mL. The biosensor can also be used for quantification of the CEA concentration in real samples.