Liu Danqing, Lv Xingxing, Zhao Chaoyue, Li Jiayue, Huang Jinmei, Weng Ling, He Liangcan, Liu Shaoqin
School of Material Science and Chemical Engineering, Harbin University of Science and Technology Harbin 150040 China
Key Laboratory of Micro-systems and Micro-structures Manufacturing of Ministry of Education, Harbin Institute of Technology Harbin 150001 China
RSC Adv. 2022 Oct 24;12(46):30174-30180. doi: 10.1039/d2ra05217b. eCollection 2022 Oct 17.
Foodborne or water-borne pathogens pose great threats to human beings and animals. There is an urgent need to detect pathogens with cheap, rapid and sensitive point-of-care diagnostic assays. Herein, we report the electrochemiluminescent (ECL) behaviors of NaBiF : Yb/Er upconversion nanoparticles (UCNPs) which were synthesized a fast and environment-friendly method at room temperature for the first time. The UCNPs together with KSO exhibit high ECL intensity and stable cathodic signals. Further, the Au nanoparticles (Au NPs) and Anti- O157 : H7 antibody were assembled on the surface of UCNPs successively to construct a novel ECL immunosensor for the detection of deadly O157 : H7. The as-prepared ECL immunosensor reveals high sensitivity to O157 : H7 in a linear range of 200-100 000 CFU mL, and the minimum detection limit could reach up to 138 CFU mL. The designed UCNP-based biosensor demonstrates high specificity, good stability and remarkable repeatability, and the strategy will provide a sensitive and selective method for rapid detection of O157 : H7 in food safety and preclinical diagnosis.
食源性病原体或水源性病原体对人类和动物构成了巨大威胁。迫切需要通过廉价、快速且灵敏的即时诊断检测方法来检测病原体。在此，我们首次报道了在室温下通过一种快速且环境友好的方法合成的NaBiF : Yb/Er上转换纳米颗粒（UCNPs）的电化学发光（ECL）行为。UCNPs与KSO一起表现出高ECL强度和稳定的阴极信号。此外，将金纳米颗粒（Au NPs）和抗O157 : H7抗体依次组装在UCNPs表面，构建了一种用于检测致命的O157 : H7的新型ECL免疫传感器。所制备的ECL免疫传感器在200 - 100 000 CFU/mL的线性范围内对O157 : H7显示出高灵敏度，最低检测限可达138 CFU/mL。所设计的基于UCNP的生物传感器具有高特异性、良好的稳定性和显著的重复性，该策略将为食品安全和临床前诊断中O157 : H7的快速检测提供一种灵敏且选择性的方法。
