Liu Bei, Li Chunxia, Xing Bengang, Yang Piaoping, Lin Jun
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
J Mater Chem B. 2016 Jul 28;4(28):4884-4894. doi: 10.1039/c6tb00799f. Epub 2016 Jun 29.
Integrated theranostic nanosystems that combine therapeutic methods with suitable medical imaging modality are highly needed in the improvement of cancer theranostic approaches. Herein, polydopamine (PDA)-shelled NaYF:Yb,Er@NaYF:Yb nanoparticles (UCNPs) capable of loading indocyanine green (ICG) molecules via electrostatic adsorption, hydrophobic interaction and π-π stacking were successfully designed and synthesized (UCNPs@PDA-ICG, denoted as UPI) for upconversion imaging and combined photothermal/photodynamic therapy (PTT/PDT) with enhanced antitumor efficacy. We have found that ICG in this novel anticancer nanoplatform can be triggered impressively by 808-nm irradiation to produce both a photothermal effect and cytotoxic reactive oxygen species (ROS), thus achieving a good PTT/PDT synergistic effect. Notably, compared with free ICG molecules, UPI nanocomposites show much higher photostability and thermal stability. Both in vitro and in vivo experiments show that the as-obtained UPI can ablate cancer cells effectively with 808-nm irradiation, revealing their great potential as a NIR-mediated dual-modal therapeutic platform. Meanwhile, the core of UCNPs can be used for UCL imaging. All these results demonstrate great potentiality of UPI as a novel type of theranostic agent for the treatment of tumors.
在癌症诊疗方法的改进中，迫切需要将治疗方法与合适的医学成像方式相结合的集成诊疗纳米系统。在此，成功设计并合成了一种聚多巴胺（PDA）包覆的NaYF:Yb,Er@NaYF:Yb纳米颗粒（UCNPs），该纳米颗粒能够通过静电吸附、疏水相互作用和π-π堆积负载吲哚菁绿（ICG）分子（UCNPs@PDA-ICG，记为UPI），用于上转换成像以及联合光热/光动力治疗（PTT/PDT），并增强了抗肿瘤疗效。我们发现，在这种新型抗癌纳米平台中的ICG在808 nm激光照射下能够显著激发，产生光热效应和细胞毒性活性氧（ROS），从而实现良好的PTT/PDT协同效应。值得注意的是，与游离ICG分子相比，UPI纳米复合材料表现出更高的光稳定性和热稳定性。体外和体内实验均表明，所制备的UPI在808 nm激光照射下能够有效消融癌细胞，揭示了其作为近红外介导的双模态治疗平台的巨大潜力。同时，UCNPs的核心可用于上转换发光成像。所有这些结果都证明了UPI作为一种新型肿瘤诊疗剂治疗肿瘤的巨大潜力。
