Yang Yulian, Qiu Yonghao, Xu Shijing, Gao Huichang, Wang Chunhui, Huang Haohui, Yang Zhengyu, Chen Xiaofeng, Zhao Fujian
Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, 510280, PR China.
School of Medicine, South China University of Technology, Guangzhou, 510006, PR China.
Bioact Mater. 2025 Mar 22;49:564-575. doi: 10.1016/j.bioactmat.2025.03.015. eCollection 2025 Jul.
Vascular injury and some chronic ischemic lesions usually lead to insufficient blood supply to tissues, which will lead to tissue ischemia or even necrosis in severe cases. Current artificial blood vessels lack effective collateral vascularization capabilities to provide adequate blood supply in areas with restricted blood flow. Herein, inspired by the grafting of tree buds to form lateral branches, the vascular buds model was successfully constructed by inoculating HUVECs into bioactive hydrogel microspheres. Under the influence of ions dissolved from bioactive glass and three-dimensional culture environment, the cytoskeleton was remodeled, the cells showed obvious outward migration and budding trend, which significantly enhanced the angiogenesis ability. After grafted vascular buds to the lateral wall of the artificial blood vessel, a large number of collateral vessels are formed, which effectively alleviates the tissue ischemia in the region through which blood vessels pass. This study confirms the impact of bioactive ions on angiogenesis in a three-dimensional environment and offers novel insights for the development of lateral branches in artificial blood vessels.
血管损伤和一些慢性缺血性病变通常会导致组织血液供应不足，严重时会导致组织缺血甚至坏死。目前的人工血管缺乏有效的侧支血管生成能力，无法在血流受限的区域提供充足的血液供应。在此，受嫁接树芽形成侧枝的启发，通过将人脐静脉内皮细胞（HUVECs）接种到生物活性水凝胶微球中，成功构建了血管芽模型。在生物活性玻璃溶解的离子和三维培养环境的影响下，细胞骨架发生重塑，细胞呈现出明显的向外迁移和出芽趋势，显著增强了血管生成能力。将血管芽移植到人工血管侧壁后，形成了大量侧支血管，有效缓解了血管所经过区域的组织缺血。本研究证实了生物活性离子在三维环境中对血管生成的影响，并为人工血管侧支的发展提供了新的见解。
