Dong Xixi, Li Haiyan, Zhou Yanling, Ou Long, Cao Junkai, Chang Jiang
Stomatology Department, General Hospital of Chinese PLA, 28 Fu Xing Road, Beijing 100853, China.
J Mater Chem B. 2016 Apr 7;4(13):2369-2376. doi: 10.1039/c6tb00398b. Epub 2016 Mar 16.
Induced pluripotent stem cells (iPSCs) have great potential as seed cells for tissue engineering applications. Previous studies have shown that iPSCs could be induced to differentiate into bone forming cells. However, in a tissue engineering approach, seeding cells in biomaterials is required, and the effect of biomaterials on cell growth and differentiation is critical for the success of the formation of engineered tissues. In this study, we investigated the effect of akermanite, a bioactive ceramic, on the osteogenic differentiation of embryoid body (EB) cells derived from human iPSCs. The results showed that, in the presence of osteogenic factors (ascorbic acid, dexamethasone, and β-glycerophosphate), ionic extracts of akermanite enhanced the osteogenic differentiation of EB cells as compared with normal osteogenic medium. Alkaline phosphatase (ALP) activity and the expression of osteogenic marker genes such as osteocalcin (OCN), collagen (COL-1), RUNX2, and BMP2 are significantly increased by the stimulation of akermanite ceramic extracts at certain concentration ranges. More interesting is that the medium containing extracts of akermanite but without osteogenic factors also showed stimulatory effects on the osteogenic differentiation of EB cells as compared to normal growth medium without osteogenic factors, such as ascorbic acid, dexamethasone, and β-glycerophosphate, not at the early stage of culture, but only at the later stage of the culture period (21 days). These results suggest that akermanite as a bioactive material together with human iPSCs might be used for bone tissue engineering applications.
诱导多能干细胞（iPSCs）作为组织工程应用的种子细胞具有巨大潜力。先前的研究表明，iPSCs可被诱导分化为成骨细胞。然而，在组织工程方法中，需要将细胞接种到生物材料中，并且生物材料对细胞生长和分化的影响对于工程组织形成的成功至关重要。在本研究中，我们研究了生物活性陶瓷钙硅石对源自人iPSCs的胚状体（EB）细胞成骨分化的影响。结果表明，在存在成骨因子（抗坏血酸、地塞米松和β-甘油磷酸）的情况下，与正常成骨培养基相比，钙硅石的离子提取物增强了EB细胞的成骨分化。在一定浓度范围内，钙硅石陶瓷提取物的刺激显著增加了碱性磷酸酶（ALP）活性和成骨标记基因如骨钙素（OCN）、胶原蛋白（COL-1）、RUNX2和BMP2的表达。更有趣的是，与不含成骨因子（如抗坏血酸、地塞米松和β-甘油磷酸）的正常生长培养基相比，含有钙硅石提取物但不含成骨因子的培养基对EB细胞的成骨分化也显示出刺激作用，不是在培养早期，而是仅在培养后期（21天）。这些结果表明，钙硅石作为一种生物活性材料与人iPSCs一起可能用于骨组织工程应用。
