Pang Qian, Zheng Xiaowen, Luo Yu, Ma Lie, Gao Changyou
MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
J Mater Chem B. 2017 Dec 7;5(45):8975-8982. doi: 10.1039/c7tb01696d. Epub 2017 Nov 8.
The development of antibiotic-loaded wound dressings is of great importance in wound healing and infection treatment. However, in traditional antibiotic-loaded wound dressings, antibiotics are released uncontrollably from the dressings primarily by passive diffusion. The overuse of antibiotics may lead to drug resistance and side effects, which could hinder the healing process. To overcome these shortcomings, a wound dressing with UV-responsive antibacterial property was developed in this study. Levofloxacin (LF) was first conjugated to amine-terminated poly(ethylene glycol) through a photolabile ortho-nitrobenzyl linker to obtain a photo-cleavable polyprodrug (LHP), which was then loaded into a poly(vinyl alcohol)/sodium alginate (PVA/SA) wound dressing. Compared to the PVA/SA dressing, the LHP-loaded dressing showed larger swelling capacity and retained good cytocompatibility. It was found that LF could be cleaved from LHP under UV irradiation at 365 nm and released gradually from the wound dressing with an increase in UV irradiation time. The antibacterial zone test proved that the LHP-loaded wound dressing possessed UV-responsive antibacterial properties against Staphylococcus aureus.
载抗生素伤口敷料的研发在伤口愈合和感染治疗中具有重要意义。然而，在传统的载抗生素伤口敷料中，抗生素主要通过被动扩散从敷料中不受控制地释放。抗生素的过度使用可能导致耐药性和副作用，从而阻碍愈合过程。为克服这些缺点，本研究开发了一种具有紫外线响应抗菌性能的伤口敷料。首先通过光不稳定的邻硝基苄基连接子将左氧氟沙星（LF）与胺端基聚乙二醇共轭，得到可光裂解的前药聚合物（LHP），然后将其负载到聚乙烯醇/海藻酸钠（PVA/SA）伤口敷料中。与PVA/SA敷料相比，载LHP的敷料显示出更大的溶胀能力，并保留了良好的细胞相容性。研究发现，在365 nm紫外线照射下，LF可从LHP上裂解下来，并随着紫外线照射时间的增加从伤口敷料中逐渐释放。抗菌圈试验证明，载LHP的伤口敷料对金黄色葡萄球菌具有紫外线响应抗菌性能。
