Huang Ho Ying, Skripka Artiom, Zaroubi Liana, Findlay Brandon L, Vetrone Fiorenzo, Skinner Cameron, Oh Jung Kwon, Cuccia Louis A
Department of Biochemistry and Chemistry, Concordia University, Montreal, Quebec, Canada H4B 1R6.
Centre Énergie, Matériaux et Télécommunications, Institut National de la Recherche Scientifique, Université du Québec, Varennes, Quebec, Canada J3X 1S2.
ACS Appl Bio Mater. 2020 Oct 19;3(10):7219-7227. doi: 10.1021/acsabm.0c01019. Epub 2020 Oct 8.
Chronic wounds present a high risk of infection due to delayed and incomplete healing, leading to increased health risks and financial burden to health-care systems. Numerous approaches to promote wound healing have been extensively explored, especially the development of effective wound dressing materials embedded with therapeutic drug molecules. Despite advances made in this area, a remaining challenge to be addressed is the controlled, on-demand release of therapeutic molecules using noncytotoxic stimulus, for example, near-infrared (NIR) excitation. Here, we report a platform that allows for the development of electrospun poly(vinyl alcohol) (PVA) fibrous hybrids embedded with upconverting nanoparticles (UCNPs) and UV-cleavable levofloxacin conjugates for wound dressings. Upon irradiation with NIR light, the excited UCNPs emit UV light around 365 nm, which can cleave the -nitrobenzyl (ONB) linkage of the levofloxacin conjugates in the PVA fiber, leading to controlled drug release. The release was observed to be triggered only under NIR and UV irradiation, with no effect in the dark. Furthermore, the antibacterial effect against and was successfully demonstrated, highlighting the versatility of the electrospun upconverting fiber platform. The development of antibacterial fibrous meshes with on-demand release of encapsulated drugs is imperative for precise treatment of wound infections.
慢性伤口由于愈合延迟和不完全而存在高感染风险，给医疗保健系统带来了更高的健康风险和经济负担。人们已经广泛探索了多种促进伤口愈合的方法，特别是开发嵌入治疗药物分子的有效伤口敷料材料。尽管在这一领域取得了进展，但仍有待解决的挑战是使用无细胞毒性的刺激（例如近红外（NIR）激发）来控制治疗分子的按需释放。在此，我们报告了一个平台，该平台可用于开发用于伤口敷料的、嵌入上转换纳米颗粒（UCNP）和可紫外线裂解的左氧氟沙星共轭物的电纺聚乙烯醇（PVA）纤维杂化物。在用近红外光照射时，被激发的上转换纳米颗粒会发出波长约为365nm的紫外光，这可以裂解PVA纤维中左氧氟沙星共轭物的对硝基苄基（ONB）键，从而实现药物的可控释放。观察到释放仅在近红外和紫外照射下触发，在黑暗中无影响。此外，成功证明了对[具体细菌名称1]和[具体细菌名称2]的抗菌效果，突出了电纺上转换纤维平台的多功能性。开发具有按需释放封装药物功能的抗菌纤维网对于精确治疗伤口感染至关重要。
