Xu Chen, Li Qing, Hu Xin-Ting, Wang Cai-Feng, Chen Su
State Key Laboratory of Pharmaceutical Biotechnology and School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China.
J Mater Chem B. 2017 Jun 7;5(21):3816-3822. doi: 10.1039/c7tb00824d. Epub 2017 May 18.
A new cell-loading strategy is proposed to improve the self-healing properties of hydrogels prepared by free-radical polymerization. The introduction of normal human dermal fibroblast (NHDF) cells enables the formation of dually crosslinked hydrogels through hydrogen interactions between cell secretions (mainly the secretory proteins) and polymer chains. This allows an enhancement of the self-healing efficiency from 73% to 92% and an acceleration of the self-healing rate (12 times). Based on the excellent biocompatibility, antibacterial property and low toxicity, we extended the use of dually crosslinked hydrogels as wound dressings to expedite wound healing. This cell-loading concept for constructing dually crosslinked hydrogels offers an available pathway to design smart materials useful for biomedical applications.
提出了一种新的细胞加载策略，以改善通过自由基聚合制备的水凝胶的自愈性能。引入正常人皮肤成纤维细胞（NHDF）能够通过细胞分泌物（主要是分泌蛋白）与聚合物链之间的氢键相互作用形成双交联水凝胶。这使得自愈效率从73%提高到92%，并加速了自愈速率（提高了12倍）。基于优异的生物相容性、抗菌性能和低毒性，我们将双交联水凝胶作为伤口敷料的用途进行了扩展，以加速伤口愈合。这种构建双交联水凝胶的细胞加载概念为设计用于生物医学应用的智能材料提供了一条可行途径。
