Müller Werner E G, Neufurth Meik, Ackermann Maximilian, Tolba Emad, Wang Shunfeng, Feng Qingling, Schröder Heinz C, Wang Xiaohong
ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University, Duesbergweg 6, D-55128 Mainz, Germany.
J Mater Chem B. 2017 Jun 7;5(21):3823-3835. doi: 10.1039/c7tb00306d. Epub 2017 Apr 26.
We describe the fabrication of a new scaffold, an inorganic-organic hybrid biomaterial, consisting of the physiological polymers: the inorganic polymer polyphosphate (polyP), as well as the organic macromolecules collagen and chondroitin sulfate. The polyP polymer is composed of multiple phosphate orthophosphate units linked together by high-energy phosphoanhydride bonds. Chondroitin sulfate has been included due to its hydrogel-forming properties. In the presence of Ca ions, the randomly coiled polyP reorganizes together with collagen and chondroitin sulfate to linear molecules which undergo hardening. This scaffold is deposited as amorphous Ca-polyP nanoparticles (size ≈20-40 nm large) around the collagen fibers. Applying the method of freeze-extraction a stable macroporous 3D hydrogel scaffold with a Young's modulus of ≈0.4 MPa is formed comprising cavities/channels larger than 75 μm. The scaffold exhibits a pronounced capacity for induction of cell proliferation (primary human osteoblasts, phOSB) and differentiation (alkaline phosphatase gene expression). We propose that incorporation of the physiological polymer polyP into the collagen-based scaffold might allow a dynamic opening and resealing and by that facilitates the exchange of nutrients for the growth of cells. This physiological hybrid biomaterial might have the potential to be used in bone and cartilage repair.
我们描述了一种新型支架的制备方法，该支架是一种无机 - 有机杂化生物材料，由生理性聚合物组成：无机聚合物聚磷酸盐（polyP），以及有机大分子胶原蛋白和硫酸软骨素。聚P聚合物由通过高能磷酸酐键连接在一起的多个正磷酸盐单元组成。硫酸软骨素因其形成水凝胶的特性而被纳入。在钙离子存在的情况下，无规卷曲的聚P与胶原蛋白和硫酸软骨素一起重新组织成线性分子，这些分子会发生硬化。这种支架以非晶态钙 - 聚P纳米颗粒（尺寸约为20 - 40纳米）的形式沉积在胶原纤维周围。应用冷冻萃取方法形成了一种稳定的大孔三维水凝胶支架，其杨氏模量约为0.4兆帕，包含大于75微米的腔/通道。该支架具有显著的诱导细胞增殖（原代人成骨细胞，phOSB）和分化（碱性磷酸酶基因表达）的能力。我们提出，将生理性聚合物聚P掺入基于胶原蛋白的支架中可能允许动态的开放和重新封闭，从而促进营养物质的交换以利于细胞生长。这种生理性杂化生物材料可能具有用于骨和软骨修复的潜力。
