Xing Yan, Gao Qin, Zhang Yanmin, Ma Le, Loh Kang Yong, Peng Mingli, Chen Chao, Cui Yali
Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, 710069, P. R. China.
J Mater Chem B. 2017 Jun 7;5(21):3919-3926. doi: 10.1039/c7tb00637c. Epub 2017 May 10.
Nanocomposites are emerging complex nanoscale materials composed of different phases that have received considerable attention as a result of their widespread impact on a myriad of fields. Having said this, it is important to recognize that poor colloidal stabilities in physiological media and non-specific interactions with biology are key limitations prohibiting their successful translation into the development of practical technologies with real impact. Herein, PAA coated Au/FeO composite magnetic nanoparticles (NPs) with monodispersity and high biostability were engineered for the development of a highly sensitive C-reactive protein (CRP) chemiluminescence immunoassay (CLIA), based on the horseradish peroxidase (HRP)-luminol-HO system. In addition, zwitterionic glycerophosphoryl choline (GPC) was employed as a co-blocking agent to reduce the nonspecific adsorption. This assay reports a limit of detection (LOD) of 2.5 pM with high reproducibility verified clinically and demonstrating improved progress towards translation into clinics.
纳米复合材料是由不同相组成的新型复杂纳米级材料，由于其对众多领域产生广泛影响而受到了相当多的关注。话虽如此，但必须认识到，其在生理介质中的胶体稳定性差以及与生物的非特异性相互作用是关键限制因素，阻碍了它们成功转化为具有实际影响力的实用技术。在此，我们设计了具有单分散性和高生物稳定性的聚丙烯酸（PAA）包覆的金/氧化亚铁复合磁性纳米颗粒（NPs），用于开发基于辣根过氧化物酶（HRP）-鲁米诺-H₂O₂体系的高灵敏度C反应蛋白（CRP）化学发光免疫分析（CLIA）。此外，两性离子甘油磷酰胆碱（GPC）被用作共封闭剂以减少非特异性吸附。该分析方法的检测限（LOD）为2.5 pM，具有高重现性，已通过临床验证，并显示出在向临床转化方面取得了更好的进展。
