Chai Yanjun, Lin Dan, Ma Yifan, Yuan Yuan, Liu Changsheng
The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P. R. China.
J Mater Chem B. 2017 Jun 28;5(24):4633-4647. doi: 10.1039/c7tb00505a. Epub 2017 May 22.
With the worldwide rising need of severe bone defect treatment, the development of available bone substitutes with optimal mechanical strength, sustained drug release, cell affinity and osteoinductivity remains a great challenge. In this study, an rhBMP-2 loaded polymer-coated mesoporous bioactive glass (MBG) composite scaffold was developed. The uncrosslinked poly(glycerol sebacate) (PGS) or PEGylated PGS (PEGS) coating modification had enhanced the mechanical strength of the composite scaffold, solved the brittleness problem of the MBG matrix, increased the cell affinity of the material surface, and diminished the initial burst release of rhBMP-2 from mesopores of MBG. The results indicated that the PGS coating promoted the proliferation of rat bone marrow stem cells (rBMSCs), while the PEGS coating exhibited an enhancement in the osteogenic differentiation of rBMSCs. The in vivo ectopic bone formation results provide strong evidence that the rhBMP-2-loaded MBG/PEGS composite scaffolds exhibited a rapid bone forming capacity and might yield extraordinary achievements in the field of bone tissue engineering. The design considerations can be extended to other artificial scaffolds and are expected to provide new thoughts on the development of future tissue engineering materials.
随着全球对严重骨缺损治疗需求的不断增加，开发具有最佳机械强度、持续药物释放、细胞亲和力和骨诱导性的可用骨替代物仍然是一项巨大挑战。在本研究中，制备了一种负载重组人骨形态发生蛋白-2（rhBMP-2）的聚合物涂层介孔生物活性玻璃（MBG）复合支架。未交联的聚癸二酸甘油酯（PGS）或聚乙二醇化PGS（PEGS）涂层修饰提高了复合支架的机械强度，解决了MBG基质的脆性问题，增加了材料表面的细胞亲和力，并减少了rhBMP-2从MBG中孔的初始突释。结果表明，PGS涂层促进了大鼠骨髓干细胞（rBMSCs）的增殖，而PEGS涂层增强了rBMSCs的成骨分化。体内异位骨形成结果提供了有力证据，表明负载rhBMP-２的MBG/PEGS复合支架具有快速的骨形成能力，可能在骨组织工程领域取得非凡成就。这些设计考量可扩展到其他人工支架，有望为未来组织工程材料的开发提供新思路。
