Wang Shuping, Sun Changkai, Guan Shui, Li Wenfang, Xu Jianqiang, Ge Dan, Zhuang Meiling, Liu Tianqing, Ma Xuehu
Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, People's Republic of China.
J Mater Chem B. 2017 Jun 28;5(24):4774-4788. doi: 10.1039/c7tb00608j. Epub 2017 May 30.
Electroactive biomaterials are widely explored as scaffolds for nerve tissue regeneration. Poly(3,4-ethylenedioxythiophene) (PEDOT) is a conductive polymer that has been chosen to construct tissue engineered scaffolds because of its excellent conductivity and non-cytotoxicity. In the present study, an electrically conductive scaffold was prepared by assembling PEDOT on a chitosan/gelatin (Cs/Gel) porous scaffold surface via in situ interfacial polymerization. The hydrophilic Cs/Gel hydrogel was used as a template, and PEDOT nanoparticles were uniformly assembled on the scaffold surface. The static polymerization of the 3,4-ethylenedioxythiophene (EDOT) monomer at the interface between the aqueous phase and the organic phase was accompanied by the formation of the PEDOT-assembled Cs/Gel scaffolds. PEDOT/Cs/Gel scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The results confirmed the deposition of PEDOT nanoparticles with the mean diameter of 50 nm on the Cs/Gel scaffold channel surface. Compared to the Cs/Gel scaffold, the incorporation of PEDOT on the scaffold increased the electrical conductivity, hydrophilicity, mechanical properties and thermal stability, whereas decreased the water absorption and biodegradation. For biocompatibility, PEDOT/Cs/Gel scaffolds, especially the 2PEDOT/Cs/Gel scaffold group, significantly promoted neuron-like rat pheochromocytoma (PC12) cell adhesion and proliferation. The results of both the gene expression and protein level assessments suggested that the PEDOT-assembled Cs/Gel scaffold enhanced the PC12 cellular neurite growth with higher protein and gene expression levels. This is the first report on the construction of a conductive PEDOT/Cs/Gel porous scaffold via an in situ interfacial polymerization method, and the results demonstrate that it may be a promising conductive scaffold for neural tissue engineering.
电活性生物材料作为神经组织再生的支架被广泛研究。聚（3,4-乙撑二氧噻吩）（PEDOT）是一种导电聚合物，因其优异的导电性和无细胞毒性而被选用于构建组织工程支架。在本研究中，通过原位界面聚合将PEDOT组装在壳聚糖/明胶（Cs/Gel）多孔支架表面，制备了一种导电支架。亲水性Cs/Gel水凝胶用作模板，PEDOT纳米颗粒均匀地组装在支架表面。3,4-乙撑二氧噻吩（EDOT）单体在水相和有机相之间的界面处进行静态聚合，同时形成PEDOT组装的Cs/Gel支架。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和拉曼光谱对PEDOT/Cs/Gel支架进行了表征。结果证实，平均直径为50nm的PEDOT纳米颗粒沉积在Cs/Gel支架通道表面。与Cs/Gel支架相比，在支架上引入PEDOT提高了电导率、亲水性、机械性能和热稳定性，而降低了吸水率和生物降解性。在生物相容性方面，PEDOT/Cs/Gel支架，尤其是2PEDOT/Cs/Gel支架组，显著促进了大鼠嗜铬细胞瘤（PC12）细胞的神经元样黏附和增殖。基因表达和蛋白质水平评估结果均表明，PEDOT组装的Cs/Gel支架通过更高的蛋白质和基因表达水平促进了PC12细胞的神经突生长。这是首次报道通过原位界面聚合法构建导电PEDOT/Cs/Gel多孔支架，结果表明它可能是一种用于神经组织工程的有前景的导电支架。
