Emer Maiara, Cardoso Mateus Borba
Laboratório Nacional de Nanotecnologia (LNNano)/Laboratório Nacional de Luz Síncrotron (LNLS), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), CEP 13083-970, Caixa Postal 6192, Campinas, SP, Brazil.
J Mater Chem B. 2017 Oct 28;5(40):8052-8059. doi: 10.1039/c7tb01744h. Epub 2017 Sep 12.
Biological fluids contain a plethora of different components that can coat nanoparticle's surface and critically affect their interaction with living systems. The recent literature has focused efforts on understanding the overall protein role in this nanoparticle's coating (commonly called a protein corona) without considering other components' influence. Here, we report a protocol to study the relationship between biological fluid components excluding proteins (referred to as a biomolecular corona) and nanoparticles. For this purpose, functionalized silica nanoparticles were duly synthesized to experimentally investigate the extent of biomolecular corona adsorption over different surfaces. This adsorption was directly correlated with the silica surface chemical constitution, while the extent of biomolecular corona coating affected the overall surface charge and the colloidal stability of the functionalized nanoparticles. In addition, the bactericidal properties of biomolecular corona-coated nanoparticles were tested against two medically relevant bacteria (Escherichia coli and Staphylococcus aureus). Biological results indicated that the presence and the extent of the biomolecular corona affect the bactericidal properties of the nanoparticles.
生物流体包含大量不同的成分，这些成分可以覆盖纳米颗粒的表面，并严重影响它们与生物系统的相互作用。最近的文献集中精力研究蛋白质在这种纳米颗粒涂层（通常称为蛋白质冠层）中的整体作用，而没有考虑其他成分的影响。在此，我们报告了一种研究排除蛋白质的生物流体成分（称为生物分子冠层）与纳米颗粒之间关系的方案。为此，我们合成了功能化的二氧化硅纳米颗粒，以实验研究生物分子冠层在不同表面上的吸附程度。这种吸附与二氧化硅表面的化学组成直接相关，而生物分子冠层的覆盖程度影响了功能化纳米颗粒的整体表面电荷和胶体稳定性。此外，我们还测试了生物分子冠层包覆的纳米颗粒对两种医学相关细菌（大肠杆菌和金黄色葡萄球菌）的杀菌性能。生物学结果表明，生物分子冠层的存在和程度会影响纳米颗粒的杀菌性能。
