Sun Jing, Wei Dan, Yang Ke, Yang You, Liu Xiaolu, Fan Hongsong, Zhang Xingdong
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, Sichuan, China.
J Mater Chem B. 2017 Oct 28;5(40):8060-8069. doi: 10.1039/c7tb01458a. Epub 2017 Oct 3.
Biomimetic multicellular complex tissues can be obtained via 3D microfabrication approaches, although the lack of active materials that meet the needs of both molding and the maintenance of cell function is the main bottleneck in their application. The development of 3D cell culture platforms, which are suitable for multiple cell types and various microfabrication technologies, would open the way to the biomimetic construction of multicellular complex tissues. In this study, a MMP-sensitive photocrosslinkable hydrogel system based on methacrylated alginate, which is suitable for the survival of multiple cell types and can be employed in a variety of micro-manufacturing technologies, was designed for the construction of 3D microfeatures to meet both the demands of molding and the requirement for biomimetic function. By employing the characteristics of photocrosslinking and ion crosslinking, the gel system was combined with a variety of microfabrication techniques to construct biomimetic microstructures with a spatial distribution of multiple types of cell. The diversity of shapes and cell types in these assemblies, as well as the maintenance of cell activity, collectively show that the functional MMP-sensitive gel system has the potential to be used as a cell culture platform for the construction of tissue microstructures.
通过3D微制造方法可以获得仿生多细胞复合组织，尽管缺乏既能满足成型需求又能维持细胞功能的活性材料是其应用的主要瓶颈。适用于多种细胞类型和各种微制造技术的3D细胞培养平台的开发，将为多细胞复合组织的仿生构建开辟道路。在本研究中，设计了一种基于甲基丙烯酸化海藻酸盐的基质金属蛋白酶（MMP）敏感的光交联水凝胶系统，该系统适用于多种细胞类型的存活，可用于多种微制造技术，用于构建3D微特征，以满足成型需求和仿生功能要求。通过利用光交联和离子交联的特性，该凝胶系统与多种微制造技术相结合，构建了具有多种类型细胞空间分布的仿生微结构。这些组件中形状和细胞类型的多样性以及细胞活性的维持，共同表明功能性MMP敏感凝胶系统有潜力用作构建组织微结构的细胞培养平台。
