Department of Microbiology, Federal University of Minas Gerais, Belo Horizonte, Av. Antônio Carlos, 6627, 30270-901 MG, Brazil.
Department of Biochemistry and Immunology, Federal University of Minas Gerais, Av. Pres. Antônio Carlos 6627, Belo Horizonte, 31270-901 MG, Brazil.
Benef Microbes. 2020 May 11;11(3):255-268. doi: 10.3920/BM2019.0113. Epub 2020 Apr 8.
Food allergy is triggered when there is an abnormal activation of the immune system by food allergens. Currently, there is no curative therapy for this pathological condition. Due to the immunomodulatory properties of probiotics they are potential candidates as therapeutic tools for food allergy. Therefore, the aim of this study was to evaluate the probiotic effect of UFMG A-905 (905) in an model of food allergy. Probiotic effect was assessed by clinical, histological, immunological and microbiological parameters analysis. Furthermore, we also evaluated if 905 after inactivation has an effect, as well as if such an effect is dose dependent. Our results showed that oral administration of only viable 905 promotes a significant attenuation of tissue injury and myeloperoxidase (MPO) activity levels. Moreover, the treatment reduced interleukin 17 levels, and administration of the supernatant from the yeast culture also promoted a significant decrease in MPO levels. However, considering the systemic parameters, immunoglobulin (Ig)E and IgG anti-ovalbumin, which are essentials for triggering the allergic process, there was no effect, suggesting that the yeast promotes a local but not a systemic effect in the model evaluated. In addition, we found that only high doses of viable 905 were able to attenuate the signs of inflammation. In conclusion, oral administration of 905 led to a local effect that depends on the viability of the yeast.
食物过敏是由食物过敏原引起的免疫系统异常激活引起的。目前，这种病理状况没有治愈疗法。由于益生菌具有免疫调节特性，它们是治疗食物过敏的潜在候选药物。因此，本研究旨在评估 UFMG A-905（905）在食物过敏模型中的益生菌作用。通过临床、组织学、免疫学和微生物学参数分析评估益生菌作用。此外，我们还评估了失活的 905 是否具有作用，以及这种作用是否具有剂量依赖性。我们的结果表明，仅口服活菌 905 即可显著减轻组织损伤和髓过氧化物酶（MPO）活性水平。此外，该治疗降低了白细胞介素 17 水平，并且酵母培养物的上清液的给予也促进了 MPO 水平的显著降低。然而，考虑到引发过敏过程的必需的系统性参数，免疫球蛋白（Ig）E 和 IgG 抗卵清蛋白，没有任何效果，这表明酵母在评估的模型中仅促进局部而非系统性作用。此外，我们发现只有高剂量的活菌 905 才能减轻炎症迹象。总之，口服 905 导致局部作用，该作用取决于酵母的活力。
