College of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.
College of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.
Reprod Biol. 2020 Jun;20(2):175-183. doi: 10.1016/j.repbio.2020.03.005. Epub 2020 Apr 5.
Stress can induce reproductive deficits by activating the HPA and causing oxidative stress. Some studies have indicated that the neurologic diseases or disorders induced by stress could be relieved by probiotics. Whether chronic unpredictable stress (CUS)-induced reproductive deficits could be prevented by probiotics is unclear. The present experiment was designed to evaluate the effects of L. rhamnosus Gorbach-Goldin (LGG) on CUS-induced reproductive deficits. Kunming mice were divided into control, stress, and LGG groups randomly. The mice in stress and LGG groups were exposed to CUS for 40days, in the meantime, the mice in LGG group were orally administered with LGG suspension at a dose of 0.3 mL/mouse (1×10 cells/mL), and the mice in control and stress groups were orally administered with volume-equivalent sterile saline once a day. The results showed that the CUS-induced the sperm deficits including the count, motility, morphology, ultrastructure, DNA integrity, and chromatin condensation were protected by oral administration of LGG. In addition, the change of testosterone level induced by CUS was prevented by up-regulating the expressions of StAR and P450scc in the testes. Moreover, LGG could increase the activities of catalase, glutathione peroxidase, and superoxide dismutase significantly, and decrease the levels of oxidative products malondialdehyde and protein carbonyls significantly, as well as the levels of cyclooxygenase 2, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α, to block the CUS-induced inflammatory response and the oxidative stress. The results indicated that the CUS-induced male reproductive deficits could be prevented by oral administration of LGG.
应激通过激活 HPA 并导致氧化应激，从而诱发生殖功能障碍。一些研究表明，应激引起的神经疾病或障碍可以通过益生菌得到缓解。目前尚不清楚益生菌是否可以预防慢性不可预测应激（CUS）引起的生殖功能障碍。本实验旨在评估鼠李糖乳杆菌 GG（LGG）对 CUS 诱导的生殖功能障碍的影响。昆明小鼠随机分为对照组、应激组和 LGG 组。应激组和 LGG 组的小鼠接受 CUS 处理 40 天，同时 LGG 组的小鼠每天口服给予 LGG 混悬液（剂量为 0.3mL/只，1×10 细胞/mL），对照组和应激组的小鼠每天口服给予等体积无菌生理盐水。结果表明，LGG 可保护 CUS 诱导的精子缺陷，包括精子计数、活力、形态、超微结构、DNA 完整性和染色质凝聚。此外，LGG 通过上调睾丸中 StAR 和 P450scc 的表达，可预防 CUS 引起的睾酮水平变化。此外，LGG 可显著提高过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性，降低丙二醛和蛋白质羰基等氧化产物的水平，以及环氧化酶 2、白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子-α的水平，从而阻断 CUS 引起的炎症反应和氧化应激。这些结果表明，口服 LGG 可预防 CUS 引起的雄性生殖功能障碍。
