Ge Qi, Chen Liang, Yuan Yi, Liu Lanlan, Feng Fan, Lv Peng, Ma Shangshang, Chen Keping, Yao Qin
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.
Institute of Life Sciences, Jiangsu University, Zhenjiang, China.
Front Pharmacol. 2020 Mar 20;11:347. doi: 10.3389/fphar.2020.00347. eCollection 2020.
Diabetes mellitus (DM) is a chronic inflammatory disease, and the rapidly increasing DM is becoming a major problem of global public health. Traditional Chinese medicine (TCM) has a long history of treating diabetes. It has been developed and utilized because of its good efficacy and no toxic side effects. is a traditional whole grass herbal. With the continuous deepening of pharmacological research on TCM, the active ingredients of are continuously revealed, which contained the alkaloids, flavonoids, flavonoid glycosides and amino acids that have the good effects of anti-inflammatory, anti-viral and anti-diabetic. In order to further explore the targets of active ingredients and its anti-diabetic mechanism, a feasible network pharmacology analysis model based on chemical, pharmacokinetic and pharmacological data was developed by network construction method to clarify the anti-diabetic mechanism of . The present study conducted by gas chromatography-mass spectrometer (GC/MS), which identified 208 metabolites of , of which 23 ingredients may have effective pharmacological effects after absorption, distribution, metabolism, and excretion (ADME) screening. Network pharmacological analysis on the active ingredients revealed that 5-hydroxymethylfurfural in affects the insulin resistance signaling pathway by acting on GSK3B, TNF, and MAPK1, acacetin affects the diabetic pathway by acting on INSR, DPP4, and GSK3B, that regulate type 2 diabetes, non-insulin-dependent DM, and inflammatory diseases. These results successfully indicated the potential anti-diabetic mechanism of the active ingredients of .
糖尿病（DM）是一种慢性炎症性疾病，且糖尿病的迅速增加正成为全球公共卫生的一个主要问题。中医治疗糖尿病有着悠久的历史。因其疗效良好且无毒性副作用而得到发展和应用。[草药名称]是一种传统的全草类草药。随着对中药药理研究的不断深入，[草药名称]的活性成分不断被揭示，其中含有生物碱、黄酮类、黄酮苷和氨基酸，具有抗炎、抗病毒和抗糖尿病的良好作用。为了进一步探究活性成分的作用靶点及其抗糖尿病机制，通过网络构建方法建立了一种基于化学、药代动力学和药理学数据的可行的网络药理学分析模型，以阐明[草药名称]的抗糖尿病机制。本研究采用气相色谱 - 质谱联用仪（GC/MS）进行分析，鉴定出[草药名称]的208种代谢产物，其中23种成分经吸收、分布、代谢和排泄（ADME）筛选后可能具有有效的药理作用。对活性成分的网络药理学分析表明，[草药名称]中的5 - 羟甲基糠醛通过作用于GSK3B、TNF和MAPK1影响胰岛素抵抗信号通路，刺槐素通过作用于INSR、DPP4和GSK3B影响糖尿病通路，这些通路调节2型糖尿病、非胰岛素依赖型糖尿病和炎症性疾病。这些结果成功地表明了[草药名称]活性成分潜在的抗糖尿病机制。
