Zhang Anpeng, Gao Yang, Li Yuanyuan, Ruan Banpu, Yang Shenglong, Liu Chaolei, Zhang Bin, Jiang Hongzhen, Fang Guonan, Ding Shilin, Jahan Noushin, Xie Lihong, Dong Guojun, Xu Zhengjin, Gao Zhenyu, Guo Longbiao, Qian Qian
Rice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province, Shenyang, China.
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
Front Plant Sci. 2020 Mar 24;11:342. doi: 10.3389/fpls.2020.00342. eCollection 2020.
Rice ( L.) is an important cereal that provides food for more than half of the world's population. Besides grain yield, improving grain quality is also essential to rice breeders. Amylose content (AC), gelatinization temperature (GT) and gel consistency (GC) are considered to be three indicators for cooking and eating quality in rice. Using a genetic map of RILs derived from the super rice Liang-You-Pei-Jiu with high-density SNPs, we detected 3 QTLs for AC, 3 QTLs for GT, and 8 QTLs for GC on chromosomes 3, 4, 5, 6, 10, and 12. locus, an important determinator for AC and GC, resided in one QTL cluster for AC and GC, and here. And a novel major QTL on chromosome 10 was identified in both Lingshui and Hangzhou. With the BCF population derived from a CSSL harboring the segment for from 93-11 in PA64s background, it was fine mapped between two molecular markers within 181 kb region with 27 annotated genes. Quantitative real-time PCR results showed that eight genes were differentially expressed in endosperm of two parents. After DNA sequencing, only , which encodes a F-box domain containing protein, has 2 bp deletion in the exon of PA64s, resulting in a premature stop codon. Therefore, is considered to be the most likely candidate gene for associated with gel consistency. Identification of provides a new genetic resource for improvement of rice quality.
水稻（Oryza sativa L.）是一种重要的谷类作物，为世界上一半以上的人口提供食物。除了谷物产量外，提高稻米品质对水稻育种者也至关重要。直链淀粉含量（AC）、糊化温度（GT）和胶稠度（GC）被认为是衡量稻米蒸煮和食用品质的三个指标。利用源自超级稻两优培九的具有高密度单核苷酸多态性（SNP）的重组自交系（RIL）遗传图谱，我们在第3、4、5、6、10和12号染色体上检测到3个控制AC的数量性状位点（QTL）、3个控制GT的QTL和8个控制GC的QTL。一个控制AC和GC的重要决定因子位于AC和GC的一个QTL簇中。并且在陵水和杭州均鉴定出一个位于第10号染色体上的新的主效QTL。利用以PA64s为背景、携带来自93-11片段的染色体片段代换系（CSSL）衍生的回交群体（BCF），将其精细定位在两个分子标记之间181 kb区域内，该区域有27个注释基因。实时荧光定量PCR结果表明，8个基因在两个亲本的胚乳中差异表达。DNA测序后发现，只有一个编码含F-box结构域蛋白的基因在PA64s的外显子中有2 bp缺失，导致提前出现终止密码子。因此，该基因被认为是与胶稠度相关的最有可能的候选基因。该基因的鉴定为改良水稻品质提供了新的遗传资源。
