Hao Yuanyuan, Huang Fudeng, Gao Zhennan, Xu Junfeng, Zhu Ying, Li Chunshou
Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Hangzhou 310021, China.
Plants (Basel). 2023 Oct 12;12(20):3541. doi: 10.3390/plants12203541.
Besides increasing grain yield, improving rice ( L.) quality has been paid more and more attention recently. Cooking and eating quality (CEQ) is an important indicator of rice quality. Since CEQs are quantitative traits and challenging for measurement, efforts have mainly focused on two major genes, and . Chalkiness and floury endosperm significantly affect the eating quality of rice, leading to noticeable changes in CEQ. Due to the easily observable phenotype of floury endosperm, cloning single gene mutations that cause floury endosperm and evaluating changes in CEQs indirectly facilitate the exploration of the minor genes controlling CEQ. In this study, eight mutants with different degrees of floury endosperm, generated through ethylmethane sulfonate (EMS) mutagenesis, were analyzed. These mutants exhibited wide variation in starch morphology and CEQs. Particularly, the mutant showed spherical starch granules significantly increased rapid visco analyzer (RVA) indexes and urea swelling, while the mutant displayed extremely sharp starch granules and significantly decreased RVA indexes and urea swelling compared to the wild type. Additionally, these mutants still maintained correlations with certain RVA profiles, suggesting that the genes , which affect these indexes, may not undergo mutation. Cloning these mutated genes in the future, especially in and , will enhance the genetic network of rice eating quality and hold significant importance for molecular marker-assisted breeding to improve rice quality.
除了提高粮食产量外，改善水稻（L.）品质近来也越来越受到关注。蒸煮食味品质（CEQ）是水稻品质的一个重要指标。由于CEQ是数量性状且测量具有挑战性，研究主要集中在两个主要基因， 和 。垩白和粉质胚乳显著影响水稻的食味品质，导致CEQ出现明显变化。由于粉质胚乳的表型易于观察，克隆导致粉质胚乳的单基因突变并间接评估CEQ的变化有助于探索控制CEQ的微效基因。在本研究中，对通过甲基磺酸乙酯（EMS）诱变产生的八个不同程度粉质胚乳的突变体进行了分析。这些突变体在淀粉形态和CEQ方面表现出广泛的变异。特别是， 突变体显示球形淀粉粒显著增加，快速粘度分析仪（RVA）指标和尿素膨胀率升高，而 突变体与野生型相比显示淀粉粒极其尖锐，RVA指标和尿素膨胀率显著降低。此外，这些突变体与某些RVA谱仍保持相关性，表明影响这些指标的基因 、 可能未发生突变。未来克隆这些突变基因，特别是 和 中的突变基因，将增强水稻食味品质的遗传网络，对分子标记辅助育种提高水稻品质具有重要意义。
