Yan Meisi, Ye Leiguang, Feng Xinxin, Shi Runze, Sun Zhen, Li Zhigao, Liu Tong
Department of Pathology, Harbin Medical University Harbin 150081, China.
Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center TX 77030, United States.
Am J Cancer Res. 2020 Mar 1;10(3):965-974. eCollection 2020.
miR-590-3p acts as a tumor suppressor in glioblastoma multiform, medulloblastoma, hepatocellular carcinoma, and nephroblastoma. Here, we studied the role of miR-590-3p in triple-negative breast cancer (TNBC). The miR-590-3p levels in TNBC specimens were significantly lower than those in non-TNBC specimens. Overexpression of miR-590-3p significantly inhibited migration and invasion of TNBC cells and lung metastasis . Interestingly, miR-590-3p decreased the Slug mRNA and protein levels in TNBC cells, and luciferase reporter assay showed that miR-590-3p directly targeted 3'-UTR of Slug in TNBC cells. Importantly, overexpression of Slug reversed the inhibitory effect of miR-590-3p on migration and invasion of TNBC cells. Taken together, miR-590-3p inhibits TNBC migration and invasion by directly targeting Slug, suggesting a potential therapeutic effect of miR-590-3p for TNBC.
微小RNA-590-3p在多形性胶质母细胞瘤、髓母细胞瘤、肝细胞癌和肾母细胞瘤中发挥肿瘤抑制作用。在此，我们研究了微小RNA-590-3p在三阴性乳腺癌（TNBC）中的作用。三阴性乳腺癌标本中微小RNA-590-3p的水平显著低于非三阴性乳腺癌标本。微小RNA-590-3p的过表达显著抑制了三阴性乳腺癌细胞的迁移和侵袭以及肺转移。有趣的是，微小RNA-590-3p降低了三阴性乳腺癌细胞中Slug的信使核糖核酸和蛋白质水平，荧光素酶报告基因检测表明微小RNA-590-3p在三阴性乳腺癌细胞中直接靶向Slug的3'-非翻译区。重要的是，Slug的过表达逆转了微小RNA-590-3p对三阴性乳腺癌细胞迁移和侵袭的抑制作用。综上所述，微小RNA-590-3p通过直接靶向Slug抑制三阴性乳腺癌的迁移和侵袭，提示微小RNA-590-3p对三阴性乳腺癌具有潜在的治疗作用。
