College of Life Science, Sichuan Agricultural University, Ya'an, 625014, P. R. China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, P. R. China.
J Microbiol. 2020 May;58(5):367-376. doi: 10.1007/s12275-020-9493-9. Epub 2020 Mar 28.
The gut microbiome of captive primates can provide a window into their health and disease status. The diversity and composition of gut microbiota are influenced by not only host phylogeny, but also host diet. Old World monkeys (Cercopithecidae) are divided into two subfamilies: Cercopithecinae and Colobinae. The diet and physiological digestive features differ between these two subfamilies. Accordingly, highthroughput sequencing was used to examine gut microbiota differences between these two subfamilies, using data from 29 Cercopithecinae individuals and 19 Colobinae individuals raised in captivity. Through a comparative analysis of operational taxonomic units (OTUs), significant differences in the diversity and composition of gut microbiota were observed between Cercopithecinae and Colobinae. In particular, the gut microbiota of captive Old World monkeys clustered strongly by the two subfamilies. The Colobinae microbial diversity was higher than that of Cercopithecinae. Additionally, Firmicutes, Lactobacillaceae, Veillonellaceae, and Prevotella abundance were higher in Cercopithecinae, while Bacteroidetes, Ruminococcaceae, Christensenellaceae, Bacteroidaceae, and Acidaminococcaceae abundance were higher in Colobinae. PICRUSt analysis revealed that the predicted metagenomes of metabolic pathways associated with proteins, carbohydrates, and amino acids were significantly higher in Colobinae. In the context of host phylogeny, these differences between Cercopithecinae and Colobinae could reflect adaptations associated with their respective diets. This well-organized dataset is a valuable resource for future related research on primates and gut microbiota. Moreover, this study may provide useful insight into animal management practices and primate conservation.
圈养灵长类动物的肠道微生物组可以提供一个了解其健康和疾病状况的窗口。肠道微生物群落的多样性和组成不仅受宿主进化关系的影响，还受宿主饮食的影响。旧大陆猴（Cercopithecidae）分为两个亚科：长尾猴亚科（Cercopithecinae）和疣猴亚科（Colobinae）。这两个亚科的饮食和生理消化特征不同。因此，本研究使用高通量测序技术，对 29 只长尾猴亚科个体和 19 只疣猴亚科个体的肠道微生物组差异进行了研究，这些个体均为圈养个体。通过对操作分类单元（OTUs）的比较分析，观察到长尾猴亚科和疣猴亚科的肠道微生物群落多样性和组成存在显著差异。特别是，圈养旧大陆猴的肠道微生物群强烈地按两个亚科聚类。疣猴亚科的微生物多样性高于长尾猴亚科。此外，厚壁菌门、乳杆菌科、韦荣球菌科和普雷沃氏菌属丰度在长尾猴亚科中较高，而拟杆菌门、瘤胃球菌科、克里斯滕森菌科、拟杆菌科和阿克曼菌科丰度在疣猴亚科中较高。 PICRUSt 分析表明，与蛋白质、碳水化合物和氨基酸相关的代谢途径的预测宏基因组在疣猴亚科中显著较高。在宿主进化关系的背景下，长尾猴亚科和疣猴亚科之间的这些差异可能反映了与各自饮食相关的适应性。这个组织良好的数据集是未来灵长类动物和肠道微生物组相关研究的宝贵资源。此外，本研究可能为动物管理实践和灵长类动物保护提供有用的见解。
