Ishizaka Y, Tahira T, Ochiai M, Ikeda I, Sugimura T, Nagao M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Oncogene Res. 1988 Sep;3(2):193-7.
A transformant having ret-II was obtained by transfection of NIH3T3 cells with DNA from a human sigmoid colon cancer. Comparison of the restriction map of ret-II with that of proto-ret revealed that the ret-II has rearrangements both 5' and 3' to the sequence coding for the kinase domain. However, only the upstream rearrangement was consistently observed in all transformants, suggesting that the upstream rearrangement resulted in the activation of proto-ret. The recombination point and replaced sequence of ret-II were different from those previously reported for ret (Takahashi et al., 1985). In addition, ret-II does not retain region coding for transmembrane of proto-ret. This rearrangement, however, was not detected in the original tumor DNA by Southern blot analysis.
通过用人乙状结肠癌的DNA转染NIH3T3细胞获得了具有ret-II的转化体。将ret-II的限制性图谱与原ret的图谱进行比较，发现ret-II在编码激酶结构域的序列的5'和3'端均有重排。然而，在所有转化体中均一致观察到只有上游重排，这表明上游重排导致了原ret的激活。ret-II的重组位点和替换序列与先前报道的ret不同（高桥等人，1985年）。此外，ret-II不保留原ret的跨膜编码区域。然而，通过Southern印迹分析在原始肿瘤DNA中未检测到这种重排。
