Nagao M, Ishizaka Y, Nakagawara A, Kohno K, Kuwano M, Tahira T, Itoh F, Ikeda I, Sugimura T
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
Jpn J Cancer Res. 1990 Apr;81(4):309-12. doi: 10.1111/j.1349-7006.1990.tb02566.x.
We examined the expression of ret proto-oncogene (proto-ret) in surgically resected human neuroblastomas. Slot blot RNA hybridization revealed that all 29 neuroblastomas examined expressed the proto-ret, the relative intensity of the hybridization ranging from 1 to 48. No correlation was found between the level of expression of proto-ret and the clinical stage. The level of expression was also not correlated with N-myc amplification, the patient's age or the histological type of the tumor. Based on the previous finding that proto-ret expression is very rarely detected in tumor cell lines other than those of neuroblastoma, proto-ret expression was suggested to be a characteristic of neuroblastomas, and possibly to be involved in the genesis of neuroblastomas.
我们检测了手术切除的人类神经母细胞瘤中原癌基因ret（proto-ret）的表达。狭缝印迹RNA杂交显示，所检测的29例神经母细胞瘤均表达proto-ret，杂交的相对强度在1至48之间。未发现proto-ret的表达水平与临床分期之间存在相关性。表达水平也与N-myc扩增、患者年龄或肿瘤的组织学类型无关。基于之前的发现，即除神经母细胞瘤细胞系外，在其他肿瘤细胞系中很少检测到proto-ret表达，提示proto-ret表达是神经母细胞瘤的一个特征，并且可能参与神经母细胞瘤的发生。
