Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, No. 76 Nanguo Road, Beilin District, Xi'an, Shaanxi Province 710054, China.
Department of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University College of Medicine, No. 76 Nanguo Road, Beilin District, Xi'an, Shaanxi Province 710054, China.
Int J Pharm. 2020 May 30;582:119303. doi: 10.1016/j.ijpharm.2020.119303. Epub 2020 Apr 5.
Osteogenic differentiation is great significance for improving the bone regeneration. Present study evaluates the osteogenic ability of lanthanum (La) and silicate (SiO) substituted hydroxyapatite (MHAP) - polymeric composite coated surface treated titanium (Ti) implant. The bio-ceramic MHAP was synthesized by hydrothermal process with assistance of calcium alginate template. For enhance the hydrophilicity, the polymer poly (vinyl pyrrolidone) (PVP) was included in the composite by ultra-sonication method. The negative zeta potential value -9.97 mV of Ca-alg/ La, Si-HAP was observed after the incorporation of PVP in the matrix. Incorporation of minerals and PVP polymer was confirmed and analyzed by Energy Dispersive X-ray analysis (EDX), Fourier Transform Infra-Red spectroscopy (FT-IR) and Electron Microscopy techniques. A compact coating of the composite with the thickness of 448 nm on Ti surface was achieved by Electrophoretic deposition (EPD) method. The in-vitro MTT assay method and alkaline phosphate ALP activity (94% and 0.94 a.u respectively for the optimized composite) were utilized to determine the cell viability and differentiation on human Bone Marrow-Derived Stem Cells (hBMSCs). The osteogenic ability of bio-composite coated Ti in hBMSCs and in-vivo rat model has strongly suggests the fabricated Ti plate with bio-composite coatings can act as promising biomaterial for orthopedics.
成骨分化对提高骨再生能力具有重要意义。本研究评估了镧（La）和硅酸盐（SiO）取代的羟基磷灰石（MHAP）-聚合物复合涂层表面处理钛（Ti）植入物的成骨能力。生物陶瓷 MHAP 通过水热法合成，并用钙藻酸盐模板辅助。为了提高亲水性，通过超声处理方法将聚合物聚乙烯吡咯烷酮（PVP）包含在复合材料中。在基质中加入 PVP 后，Ca-alg/La、Si-HAP 的负zeta 电位值为-9.97 mV。通过能量色散 X 射线分析（EDX）、傅里叶变换红外光谱（FT-IR）和电子显微镜技术证实和分析了矿物质和 PVP 聚合物的掺入。通过电泳沉积（EPD）方法在 Ti 表面上获得了厚度为 448nm 的复合涂层。体外 MTT 测定法和碱性磷酸酶 ALP 活性（优化复合体系的细胞活力和分化分别为 94%和 0.94 a.u）用于确定人骨髓间充质干细胞（hBMSCs）上的细胞活力和分化。生物复合材料涂层 Ti 在 hBMSCs 和体内大鼠模型中的成骨能力强烈表明，所制备的具有生物复合材料涂层的 Ti 板可用作有前途的骨科生物材料。
