Silva Vanessa, Sousa Telma de, Gómez Paula, Sabença Carolina, Vieira-Pinto Madalena, Capita Rosa, Alonso-Calleja Carlos, Torres Carmen, Capelo José L, Igrejas Gilberto, Poeta Patrícia
Functional Genomics and Proteomics Unit, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Foods. 2020 Apr 6;9(4):439. doi: 10.3390/foods9040439.
Methicillin-resistant (MRSA) are one of the main pathogens associated with purulent infections. MRSA clonal complex 97 (CC97) has been identified in a wide diversity of livestock animals. Therefore, we aimed to investigate the antibiotic resistance profiles of MRSA strains isolated from purulent lesions of food-producing rabbits. Samples from purulent lesions of 66 rabbits were collected in a slaughterhouse in Portugal. Samples were seeded onto ORSAB plates with 2 mg/L of oxacillin for MRSA isolation. Susceptibility to antibiotics was tested by the disk diffusion method against 14 antimicrobial agents. The presence of resistance genes, virulence factors and the immune evasion cluster (IEC) system was studied by polymerase chain reaction. All isolates were characterized by multilocus sequence typing (MLST), and typing. From the 66 samples analyzed, 16 (24.2%) MRSA were detected. All strains were classified as multidrug-resistant as they were resistant to at least three classes of antibiotics. All isolates showed resistance to penicillin, erythromycin and clindamycin. Seven isolates were resistant to gentamicin and harbored the (6')-Ie- (2'')-Ia gene. Resistance to tetracycline was detected in 10 isolates harboring the (K) gene. The IEC genes were detected in three isolates. MRSA strains belonged to CC97, CC1, CC5, CC15 or CC22. The isolates were assigned to six different types. In this study we found a moderate prevalence of multidrug-resistant MRSA strains in food-producing rabbits. This may represent concern for food safety and public health, since cross-contamination may occur, leading to the spread of MRSA and, eventually, the possibility of ingestion of contaminated meat.
耐甲氧西林金黄色葡萄球菌（MRSA）是引起化脓性感染的主要病原体之一。已在多种家畜中鉴定出MRSA克隆复合体97（CC97）。因此，我们旨在调查从产肉兔化脓性病变中分离出的MRSA菌株的抗生素耐药谱。在葡萄牙的一家屠宰场收集了66只兔子化脓性病变的样本。将样本接种到含2 mg/L苯唑西林的ORSAB平板上以分离MRSA。采用纸片扩散法针对14种抗菌药物测试对抗生素的敏感性。通过聚合酶链反应研究耐药基因、毒力因子和免疫逃避簇（IEC）系统的存在情况。所有分离株均通过多位点序列分型（MLST）和分型进行鉴定。在分析的66个样本中，检测到16株（24.2%）MRSA。所有菌株均被归类为多重耐药，因为它们对至少三类抗生素耐药。所有分离株均对青霉素、红霉素和克林霉素耐药。7株分离株对庆大霉素耐药并携带（6'）-Ie-（2''）-Ia基因。在10株携带（K）基因的分离株中检测到对四环素的耐药性。在3株分离株中检测到IEC基因。MRSA菌株属于CC97、CC1、CC5、CC15或CC22。这些分离株被分为六种不同的型别。在本研究中，我们发现产肉兔中多重耐药MRSA菌株的流行率中等。这可能引起对食品安全和公共卫生的关注，因为可能发生交叉污染，导致MRSA传播，并最终导致摄入受污染肉类的可能性。
