Hotoda H, Wada T, Sekine M, Hata T
Department of Life Chemistry, Tokyo Institute of Technology, Yokohama, Japan.
Nucleic Acids Symp Ser. 1988(19):17-20.
The title compound (BDCP) was found to be effective for the rapid internucleotidic bond formation in the presence of 3-nitro-1,2,4-triazole (NT). Mechanism of the condensation reaction was elucidated by means of 31P-NMR. It was proved that there was no active species but the desired nucleotide derivatives, phosphorochloridate and phosphorylnitrotriazole, in the reaction mixture. Thus, the side-reaction of excess condensing agent in the conventional phosphotriester method was overcome.
发现标题化合物（BDCP）在3-硝基-1,2,4-三唑（NT）存在下可有效促进核苷酸间快速形成键。通过31P-NMR阐明了缩合反应的机理。结果证明，反应混合物中不存在活性物种，仅存在所需的核苷酸衍生物、氯代磷酸酯和磷酰基硝基三唑。因此，克服了传统磷酸三酯法中过量缩合剂的副反应。
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