通过使用新型缩合试剂，磷酸三酯法实现核苷酸间快速成键。
A rapid internucleotide bond formation for the phosphotriester approach by use of new condensing reagents.
作者信息
Matsuzaki J, Hotoda H, Sekine M, Hata T
出版信息
Nucleic Acids Symp Ser. 1984(15):93-6.
PMID:6522301
Abstract
Bis(2,4,6-trihalophenyl) phosphorochloridates (TCP and TBP) were found to be effective as condensing reagents for the rapid internucleotidic phoshotriester bond formation. The benzoyl group as the N3-imide protecting group of thymidine was also useful for prevention of the side reaction caused by the active condensing reagents.
摘要
双（2,4,6-三卤苯基）磷酰氯（TCP和TBP）被发现是用于快速形成核苷酸间磷酸三酯键的有效缩合试剂。苯甲酰基作为胸苷的N3-酰亚胺保护基，对于防止活性缩合试剂引起的副反应也很有用。
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