微小RNA-519通过下调FOXQ1抑制胃癌细胞的上皮-间质转化和生物学行为。
miR-519 inhibits epithelial-mesenchymal transition and biologic behavior of gastric cancer cells by down-regulating FOXQ1.
作者信息
Xu Jiapeng, You Qing, Wei Ziran, Fu Hongbing, Zhang Yu, Hu Zunqi, Cai Qingping
机构信息
Department of Gastrointestinal Surgery, Changzheng Hospital, Second Military Medical University Shanghai, China.
出版信息
Int J Clin Exp Pathol. 2020 Mar 1;13(3):425-436. eCollection 2020.
In recent years, a number of studies have shown that forkhead box Q1 (FOXQ1) plays an important role in the process of epithelial-mesenchymal transition (EMT) of tumors. The aim of this study is to investigate the biologic functions of FOXQ1 and miR-519 in gastric cancer. It was found that FOXQ1 was highly expressed in gastric cancer cells and tumor tissues, and promoted proliferation, migration, invasion, and EMT of gastric cancer cells. miR-519 was weakly expressed in both gastric cancer tissues and gastric cancer cells, up-regulation of miR-519 inhibited the biologic behavior of gastric cancer cells, while down-regulation of miR-519 showed the opposite results. Additionally, miR-519 directly targeted FOXQ1 and inhibited FOXQ1 mRNA and protein expression. Overexpression of FOXQ1 in gastric cancer cells reversed the inhibitory effect of miR-519 on cellular biologic behavior. The results of the present study suggest that the abnormal expression of miR-519 and FOXQ1 may be closely related to gastric cancer development, and miR-519 may play an important role in suppressing tumor related genes in gastric cancer by targeting and regulating FOXQ1.
近年来，多项研究表明，叉头框Q1（FOXQ1）在肿瘤上皮-间质转化（EMT）过程中发挥重要作用。本研究旨在探讨FOXQ1和miR-519在胃癌中的生物学功能。研究发现，FOXQ1在胃癌细胞和肿瘤组织中高表达，促进胃癌细胞的增殖、迁移、侵袭及EMT。miR-519在胃癌组织和胃癌细胞中均低表达，上调miR-519可抑制胃癌细胞的生物学行为，而下调miR-519则产生相反结果。此外，miR-519直接靶向FOXQ1并抑制FOXQ1的mRNA和蛋白表达。在胃癌细胞中过表达FOXQ1可逆转miR-519对细胞生物学行为的抑制作用。本研究结果提示，miR-519和FOXQ1的异常表达可能与胃癌发生密切相关，且miR-519可能通过靶向调控FOXQ1在抑制胃癌相关基因方面发挥重要作用。
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