Wu Kaiming, Zhao Zhenxian, Ma Jun, Chen Jianhui, Peng Jianjun, Yang Shibin, He Yulong
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China.
Department of Pancreato-Biliary Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510080, P.R. China.
Oncol Lett. 2017 Apr;13(4):2557-2562. doi: 10.3892/ol.2017.5763. Epub 2017 Feb 22.
MicroRNA-193b (miRNA-193b) is often differentially expressed and is an important regulator of gene expression in colon cancer. The aim of the present study was to determine whether miRNA-193b affects cell growth in colon cancer and to investigate the potential underlying mechanisms. Patients with colorectal cancer (CRC; n=20) and healthy volunteers (n=10) were enrolled from the Department of Gastrointestinal Surgery Center, First Affiliated Hospital of Sun Yat-Sen University (Guangzhou, China). Western blot analysis was used to evaluate the protein expression of SMAD3 and transforming growth factor-β (TGF-β) in the patient samples. It was determined that miRNA-193b expression was markedly elevated in the CRC tissue samples. Furthermore, silencing of miRNA-193bin SW620 CRC cells by specific inhibitors significantly reduced the cell proliferation and induced apoptosis. In addition, the downregulation of miRNA-193b significantly activated the protein expression of SMAD3 and TGF-β, and promoted caspase-3 activity in SW620 cells. The results of the present study suggested that the deregulation of miRNA-193b may affect cell growth in colon cancer via the TGF-β and SMAD3 signaling pathways.
微小RNA-193b（miRNA-193b）常常存在差异表达，是结肠癌中基因表达的重要调节因子。本研究旨在确定miRNA-193b是否影响结肠癌细胞的生长，并探究其潜在的作用机制。研究对象来自中山大学附属第一医院胃肠外科中心（中国广州），纳入了20例结直肠癌（CRC）患者和10例健康志愿者。采用蛋白质印迹分析评估患者样本中SMAD3和转化生长因子-β（TGF-β）的蛋白表达。结果显示，CRC组织样本中miRNA-193b的表达显著升高。此外，用特异性抑制剂沉默SW620 CRC细胞中的miRNA-193b可显著降低细胞增殖并诱导细胞凋亡。另外，miRNA-193b的下调显著激活了SW620细胞中SMAD3和TGF-β的蛋白表达，并促进了caspase-3的活性。本研究结果表明，miRNA-193b的失调可能通过TGF-β和SMAD3信号通路影响结肠癌细胞的生长。
