Liu Rui, Cao Ke, Tang Yuanyuan, Liu Jinyan, Li Jingjing, Chen Jia, Wang Shaohua, Chen Zizi, Zhou Jianda
Department of Orthopedics, Xiangya Hospital of Central South University Changsha, P. R. China.
Department of Oncology, Third Xiangya Hospital of Central South University Changsha, P. R. China.
Am J Transl Res. 2020 Mar 15;12(3):1123-1135. eCollection 2020.
To investigate the role of C16:0 ceramide in melanoma metastatic behavior and glycolysis, five common long-chain ceramides (C16:0, C18:0, C20:0, C22:0, C24:0) were tested in melanocyte and melanoma cell lines by LC-MS. We then treated non-metastatic and metastatic melanoma cells with PDMP and exogenous C16:0 to explore their effects on proliferation, migration, and glycolysis. The long-chain ceramide was also analyzed by LC-MS after treatment. C16:0 ceramide showed the highest levels in melanocyte and melanoma cells, with all melanomas higher than melanocytes. PDMP inhibited malignant behavior and glycolysis in melanoma, and caused the accumulation of intracellular C16:0. Exogenous C16:0 promoted melanoma glycolysis, but not malignant behavior, and decreased intracellular C16:0. Finally, pyruvate kinase (PK), hexokinase (HK), and lactic acid dehydrogenase (LDH) activity, key enzymes in glycolysis, were altered after treatment with PDMP and exogenous C16:0.
为研究C16:0神经酰胺在黑色素瘤转移行为和糖酵解中的作用，通过液相色谱-质谱联用（LC-MS）在黑素细胞和黑色素瘤细胞系中检测了五种常见的长链神经酰胺（C16:0、C18:0、C20:0、C22:0、C24:0）。然后用PDMP和外源性C16:0处理非转移性和转移性黑色素瘤细胞，以探究它们对增殖、迁移和糖酵解的影响。处理后还通过LC-MS分析了长链神经酰胺。C16:0神经酰胺在黑素细胞和黑色素瘤细胞中的水平最高，所有黑色素瘤中的水平均高于黑素细胞。PDMP抑制黑色素瘤的恶性行为和糖酵解，并导致细胞内C16:0的积累。外源性C16:0促进黑色素瘤糖酵解，但不促进恶性行为，并降低细胞内C16:0。最后，用PDMP和外源性C16:0处理后，糖酵解中的关键酶丙酮酸激酶（PK）、己糖激酶（HK）和乳酸脱氢酶（LDH）的活性发生了改变。
