Fursova Ksenia, Sorokin Anatoly, Sokolov Sergey, Dzhelyadin Timur, Shulcheva Irina, Shchannikova Margarita, Nikanova Daria, Artem'eva Olga, Zinovieva Natalia, Brovko Fedor
Laboratory of Immunochemistry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Russia.
Laboratory of Cell Genome Functioning Mechanisms, Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino, Russia.
Front Vet Sci. 2020 Mar 25;7:135. doi: 10.3389/fvets.2020.00135. eCollection 2020.
is a causative agent of different infectious processes, food poisoning, and autoimmune disorders. The horizontal transfer of pathogenic strains can occur from animal to human under both house and farm conditions, and the spread of strains with antibiotic resistance is an existing problem. In addition to the spread of antibiotic-resistant strains in clinics, this problem also exists in veterinary medicine. It is especially important to monitor antibiotic resistance on farms where antibiotics are the standard treatment of animals, which may trigger the spread of antibiotic-resistant strains among animals and to the human population, and these strains can also be distributed in milk products produced by these farms (milk, cheese, and butter). In this work, we investigated 21 isolates using whole-genome sequence analysis and tried to establish a relationship between these isolates with the development of bovine mastitis in seven regions of Western Russia. An virulence profile was identified. We identified two groups of associated with subclinical mastitis, namely, the enterotoxin-positive and enterotoxin-negative groups. The most prevalent factor associated with bovine mastitis in Russia was cytotoxins, including hemolysins and leukocidins. Multidrug resistance strains were investigated, and antibiotic resistance genes were identified. We identified ST 97 type as the most common type in the regions in Western Russia. To the best of our knowledge, this is the first in-depth study of a range isolates originating from cattle infections in Russia.
是不同感染过程、食物中毒和自身免疫性疾病的病原体。致病菌株的水平传播可在家庭和农场条件下从动物传播给人类，且具有抗生素抗性的菌株传播是一个现存问题。除了在诊所中抗生素抗性菌株的传播，这个问题在兽医学中也存在。在抗生素是动物标准治疗手段的农场监测抗生素抗性尤为重要，这可能引发抗生素抗性菌株在动物之间以及向人类的传播，并且这些菌株还可分布在这些农场生产的奶制品（牛奶、奶酪和黄油）中。在这项工作中，我们使用全基因组序列分析研究了21株分离株，并试图在俄罗斯西部七个地区的这些分离株与牛乳腺炎的发生之间建立联系。确定了一种毒力谱。我们鉴定出两组与亚临床乳腺炎相关的菌株，即肠毒素阳性组和肠毒素阴性组。在俄罗斯，与牛乳腺炎相关的最普遍因素是细胞毒素，包括溶血素和杀白细胞素。对多重耐药菌株进行了研究，并鉴定出抗生素抗性基因。我们确定ST 97型是俄罗斯西部各地区最常见的类型。据我们所知，这是对源自俄罗斯牛感染的一系列分离株的首次深入研究。
