Bardasheva Alevtina, Tikunov Artem, Kozlova Yuliya, Zhirakovskaia Elena, Fedorets Valeriya, Fomenko Natalya, Kalymbetova Tatyana, Chretien Svetlana, Pavlov Vitaliy, Tikunova Nina, Morozova Vera
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, 630090 Novosibirsk, Russia.
Joint-Stock Company Vector-Best, P.O. Box 121, 630117 Novosibirsk, Russia.
Microorganisms. 2021 Nov 30;9(12):2487. doi: 10.3390/microorganisms9122487.
A total of 394 strains of staphylococci found in humans and pets in Novosibirsk, Siberian Russia, were characterized in terms of antibiotic resistance and corresponding genes. Two coagulase-positive and 17 coagulase-negative species were identified. The majority of isolates, with the exception of and hospital isolates, were sensitive to most of the tested antibiotics, and isolates from pets displayed the lowest level of resistance. Nevertheless, methicillin-resistant (MRS) and/or multidrug-resistant (MDR) isolates were found in all prevailed species, including coagulase-negative. A set of genes corresponding to the detected resistance was identified: (beta-lactam resistance), (aminoglycoside-modifying enzymes), , and (macrolide resistance). Complete genome analysis for ten MDR and five MDR isolates revealed additional antibiotic resistance genes , , , , , , and . , , and were present in all genomes, whereas and were identified in all ones. All investigated MDR and four of five strains were moderate or strong biofilm producers, whereas multiple genes responsible for this function and for virulence and pathogenicity were identified mostly in , but were less frequently represented in .
对在俄罗斯西伯利亚新西伯利亚的人类和宠物身上发现的总共394株葡萄球菌进行了抗生素耐药性及相应基因方面的特征分析。鉴定出了2种凝固酶阳性和17种凝固酶阴性菌种。除[未提及的菌种]和医院分离株外，大多数分离株对大多数测试抗生素敏感，宠物分离株的耐药水平最低。然而，在所有优势菌种中，包括凝固酶阴性菌种，均发现了耐甲氧西林（MRS）和/或多重耐药（MDR）分离株。鉴定出了一组与检测到的耐药性相对应的基因：[未提及具体基因名，可能是β-内酰胺耐药相关基因]（β-内酰胺耐药）、[未提及具体基因名，可能是氨基糖苷类修饰酶相关基因]（氨基糖苷类修饰酶）、[未提及具体基因名，可能是大环内酯耐药相关基因]和[未提及具体基因名，可能是大环内酯耐药相关基因]（大环内酯耐药）。对10株MDR[未提及具体菌种名]和5株MDR[未提及具体菌种名]分离株进行的全基因组分析揭示了额外的抗生素耐药基因[未提及具体基因名]、[未提及具体基因名]、[未提及具体基因名]、[未提及具体基因名]、[未提及具体基因名]、[未提及具体基因名]和[未提及具体基因名]。[未提及具体基因名]、[未提及具体基因名]和[未提及具体基因名]存在于所有[未提及具体菌种名]基因组中，而[未提及具体基因名]和[未提及具体基因名]在所有[未提及具体菌种名]基因组中被鉴定出来。所有调查的MDR[未提及具体菌种名]和5株[未提及具体菌种名]菌株中的4株是中度或强生物膜产生菌，而负责此功能以及毒力和致病性的多个基因大多在[未提及具体菌种名]中被鉴定出来，但在[未提及具体菌种名]中出现的频率较低。
